Western Blot (WB)
(Western Blot; Sample: Recombinant NUMA1, Human.)
Rabbit anti-Human Nuclear Mitotic Apparatus Protein 1 Polyclonal Antibody | anti-NUMA1 antibody
Polyclonal Antibody to Nuclear Mitotic Apparatus Protein 1 (NUMA1)
Immunohistochemistry: 5-20ug/mL; 1:20-80
Immunocytochemistry: 5-20ug/mL; 1:20-80
Optimal working dilutions must be determined by end user.
Store at 4°C for frequent use.
Aliquot and store at -20°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot; Sample: Recombinant NUMA1, Human.)
Western Blot (WB)
(Western Blot; Sample: Recombinant NUMA1, Human.)
Western Blot (WB)
(Western Blot; Sample: Human A431 cell lysate;Primary Ab: 2ug/ml Rabbit Anti-Human NUMA1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Western Blot (WB)
(Western Blot; Sample: Human A431 cell lysate;Primary Ab: 2ug/ml Rabbit Anti-Human NUMA1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
Uniprot Description
NuMA-1: a coiled-coil nuclear protein that is required to establish and maintain mammalian spindle poles. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. Two human splice-variant isoforms have been described.
Protein type: Cell cycle regulation; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 11q13.4
Cellular Component: apical part of cell; cell cortex; cell soma; centrosome; chromosome; cytoplasmic microtubule bundle; cytosol; dendrite; extracellular exosome; extrinsic component of plasma membrane; Golgi membrane; lateral plasma membrane; microtubule minus-end; mitotic spindle midzone; nuclear matrix; nucleoplasm; nucleus; protein complex; spindle; spindle microtubule; spindle pole; spindle pole centrosome
Molecular Function: microtubule binding; microtubule minus-end binding; microtubule plus-end binding; phosphatidylinositol binding; protein binding; protein C-terminus binding; protein complex binding; protein domain specific binding; structural molecule activity; tubulin binding
Biological Process: anastral spindle assembly; astral microtubule organization; cell division; chromosome segregation; establishment of mitotic spindle orientation; meiotic cell cycle; microtubule bundle formation; nucleus organization; positive regulation of BMP signaling pathway; positive regulation of chromosome segregation; positive regulation of hair follicle development; positive regulation of intracellular transport; positive regulation of keratinocyte differentiation; positive regulation of microtubule polymerization; positive regulation of protein localization to cell cortex; positive regulation of protein localization to spindle pole body; regulation of metaphase plate congression
Disease: Acute Promyelocytic Leukemia
Research Articles on NUMA1
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Product Notes
The NUMA1 numa1 (Catalog #AAA2026863) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Nuclear Mitotic Apparatus Protein 1 (NUMA1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nuclear Mitotic Apparatus Protein 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL; 1:200-800 Immunohistochemistry: 5-20ug/mL; 1:20-80 Immunocytochemistry: 5-20ug/mL; 1:20-80 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the NUMA1 numa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nuclear Mitotic Apparatus Protein 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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