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Western Blot (WB) (Western Blot analysis of COS7 cells using NUB1 Polyclonal Antibody)

Rabbit NUB1 Polyclonal Antibody | anti-NUB1 antibody

NUB1 Polyclonal Antibody

Gene Names
NUB1; BS4; NUB1L; NYREN18
Reactivity
Human, Mouse, Monkey
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
NUB1; Polyclonal Antibody; NUB1 Polyclonal Antibody; NYREN18; NEDD8 ultimate buster 1; Negative regulator of ubiquitin-like proteins 1; Renal carcinoma antigen NY-REN-18; anti-NUB1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Monkey
Clonality
Polyclonal
Specificity
NUB1 Polyclonal Antibody detects endogenous levels of NUB1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
639
Applicable Applications for anti-NUB1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:100 - 1:300
ELISA: 1:20000
Not yet tested in other applications.
Immunogen Type
peptide
Immunogen Description
Synthesized peptide derived from the C-terminal region of human NUB1.
Target Name
NUB1
Preparation and Storage
Store at -20 degree C/1 year

Western Blot (WB)

(Western Blot analysis of COS7 cells using NUB1 Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of COS7 cells using NUB1 Polyclonal Antibody)
Product Categories/Family for anti-NUB1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
69,119 Da
NCBI Official Full Name
NEDD8 ultimate buster 1 isoform 1
NCBI Official Synonym Full Names
negative regulator of ubiquitin-like proteins 1
NCBI Official Symbol
NUB1
NCBI Official Synonym Symbols
BS4; NUB1L; NYREN18
NCBI Protein Information
NEDD8 ultimate buster 1
UniProt Protein Name
NEDD8 ultimate buster 1
Protein Family
UniProt Gene Name
NUB1
UniProt Synonym Gene Names
NYREN18
UniProt Entry Name
NUB1_HUMAN

NCBI Description

This gene encodes a protein that functions as a negative regulator of NEDD8, a ubiquitin-like protein that conjugates with cullin family members in order to regulate vital biological events. The protein encoded by this gene regulates the NEDD8 conjugation system post-transcriptionally by recruiting NEDD8 and its conjugates to the proteasome for degradation. This protein interacts with the product of the AIPL1 gene, which is associated with Leber congenital amaurosis, an inherited retinopathy, and mutations in that gene can abolish interaction with this protein, which may contribute to the pathogenesis. This protein is also known to accumulate in Lewy bodies in Parkinson's disease and dementia with Lewy bodies, and in glial cytoplasmic inclusions in multiple system atrophy, with this abnormal accumulation being specific to alpha-synucleinopathy lesions. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Uniprot Description

NYREN18: Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: cytoplasm; nucleolus; nucleus

Molecular Function: protein binding

Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination

Research Articles on NUB1

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Product Notes

The NUB1 nub1 (Catalog #AAA9413044) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NUB1 Polyclonal Antibody reacts with Human, Mouse, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's NUB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100 - 1:300 ELISA: 1:20000 Not yet tested in other applications. Researchers should empirically determine the suitability of the NUB1 nub1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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