Rabbit anti-Human NT-proBNP Polyclonal Antibody | anti-NT-proBNP antibody

Rabbit anti-human NT-proBNP polyclonal Antibody

Cat. Num. AAA715412

• Gene Names: NPPB; BNP
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%, Protein G purified
• Synonyms: NT-proBNP; Polyclonal Antibody; Rabbit anti-human NT-proBNP polyclonal Antibody; Natriuretic peptides B (Gamma-brain natriuretic peptide) [Cleaved into: Brain natriuretic peptide 32 (BNP(1-32)) (BNP-32); BNP(1-30); BNP(1-29); BNP(1-28); BNP(2-31); BNP(3-32); BNP(3-30); BNP(3-29); BNP(4-32); BNP(4-31); BNP(4-30); BNP(4-29); BNP(4-27); BNP(5-32); BNP(5-31); BNP(5-29)]; NPPB; anti-NT-proBNP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid

• Applicable Applications for anti-NT-proBNP antibody: ELISA, Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200
• Immunogen: Recombinant Human Natriuretic peptides B protein (27-102AA)
• Conjugate: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Species: Human
• Research Area: Signal Transduction
• Target Names: NPPB
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-18814 / sc-18813 / sc-20159
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: NT-proBNP antibody at 2ug/ml+Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 15kDaObserved band size: 15kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human brain tissue using MBS715412 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human heart tissue using MBS715412 at dilution 1:100)

Related Product Information for anti-NT-proBNP antibody

Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

NCBI and Uniprot Product Information

• NCBI GI #: 4505433
• NCBI GeneID: 4879
• NCBI Accession #: NP_002512.1
• NCBI GenBank Nucleotide #: NM_002521.2
• UniProt Accession #: P16860; Q6FGY0; Q9P2Q7; B0ZBE9
• NCBI Official Full Name: natriuretic peptides B preproprotein
• NCBI Official Synonym Full Names: natriuretic peptide B
• NCBI Official Symbol: NPPB
• NCBI Official Synonym Symbols: BNP
• NCBI Protein Information: natriuretic peptides B
• UniProt Protein Name: Natriuretic peptides B
• UniProt Gene Name: NPPB
• UniProt Synonym Gene Names: BNP(1-32); BNP-32
• UniProt Entry Name: ANFB_HUMAN

NCBI Description

This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Nov 2014]

Uniprot Description

NPPB: Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family.

Protein type: Secreted; Hormone; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: extracellular region; extracellular space; protein complex

Molecular Function: diuretic hormone activity; hormone activity; protein binding; receptor binding

Biological Process: body fluid secretion; cell surface receptor linked signal transduction; cGMP biosynthetic process; negative regulation of angiogenesis; negative regulation of cell growth; receptor guanylyl cyclase signaling pathway; regulation of blood pressure; regulation of vascular permeability; regulation of vasodilation