Western Blot (WB)
(NSMAF Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the NSMAF antibody detected the NSMAF protein (arrow).)
Rabbit anti-Mouse NSMAF Polyclonal Antibody | anti-NSMAF antibody
NSMAF Antibody (N-term)
Western Blot (WB)
(NSMAF Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the NSMAF antibody detected the NSMAF protein (arrow).)
Western Blot (WB)
(NSMAF Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the NSMAF antibody detected the NSMAF protein (arrow).)
Immunohistochemistry (IHC)
(NSMAF Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NSMAF Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(NSMAF Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NSMAF Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
cytoplasmic sphingomyelinase activation domain of the 55kD tumor
necrosis factor receptor. This protein is required for TNF-mediated
activation of neutral sphingomyelinase and may play a role in
regulating TNF-induced cellular responses such as inflammation.
Alternative splicing results in multiple transcript variants.
Need, A.C., et al. Hum. Mol. Genet. 18(23):4650-4661(2009)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Clarke, C.J., et al. Mol. Pharmacol. 74(4):1022-1032(2008)
Kolzer, M., et al. Biol. Chem. 385(12):1193-1195(2004)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]
Uniprot Description
NSMAF: Couples the p55 TNF-receptor (TNF-R55 / TNFR1) to neutral sphingomyelinase (N-SMASE). Specifically binds to the N- smase activation domain of TNF-R55. May regulate ceramide production by N-SMASE. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold; Autophagy
Chromosomal Location of Human Ortholog: 8q12-q13
Cellular Component: extrinsic to membrane; cytoplasm; endomembrane system
Molecular Function: sphingomyelin phosphodiesterase activator activity; protein binding; receptor signaling protein activity; phospholipid binding
Biological Process: positive regulation of catalytic activity; positive regulation of apoptosis; ceramide metabolic process; signal transduction; endosome transport
Research Articles on NSMAF
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Product Notes
The NSMAF nsmaf (Catalog #AAA9203965) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 28-57. The NSMAF Antibody (N-term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NSMAF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the NSMAF nsmaf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NSMAF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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