Rabbit anti-Mouse NSF Polyclonal Antibody | anti-NSF antibody

NSF, CT (NSF, Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, Vesicular-fusion protein NSF) (MaxLight 550)

Cat. Num. AAA6326871

• Gene Names: NSF; SKD2
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NSF; Polyclonal Antibody; CT (NSF; Vesicle-fusing ATPase; N-ethylmaleimide-sensitive fusion protein; Vesicular-fusion protein NSF) (MaxLight 550); Vesicular-fusion protein NSF; anti-NSF antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-NSF antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NSF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 668-696 amino acids from the C-terminal region of human NSF.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-NSF antibody

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.

Product Categories/Family for anti-NSF antibody

Antibodies; Enzymes; ATPase

References

Saus, E., et al. J Psychiatr Res 44(14):971-978(2010). Hamza, T.H., et al. Nat. Genet. 42(9):781-785(2010). Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010): Parashuraman, S., et al. FEBS Lett. 584(6):1251-1256(2010). Simon-Sanchez, J., et al. Nat. Genet. 41(12):1308-1312(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 156564401
• NCBI GeneID: 4905
• NCBI Accession #: NP_006169.2
• NCBI GenBank Nucleotide #: NM_006178.3
• UniProt Accession #: P46459; Q8N6D7; Q9UKZ2; A8K2D9
• Molecular Weight: 82,594 Da
• NCBI Official Full Name: vesicle-fusing ATPase
• NCBI Official Synonym Full Names: N-ethylmaleimide-sensitive factor
• NCBI Official Symbol: NSF
• NCBI Official Synonym Symbols: SKD2
• NCBI Protein Information: vesicle-fusing ATPase; NEM-sensitive fusion protein; vesicular-fusion protein NSF; N-ethylmaleimide-sensitive fusion protein; N-ethylmaleimide-sensitive factor-like protein
• UniProt Protein Name: Vesicle-fusing ATPase
• Protein Family: NSFL1 cofactor
• UniProt Gene Name: NSF
• UniProt Synonym Gene Names: NEM-sensitive fusion protein
• UniProt Entry Name: NSF_HUMAN

Uniprot Description

NSF: required for intracellular membrane fusion and vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Functions as a SNAP receptor (SNARE) chaperone which binds, through soluble NSF attachment proteins (SNAPs), to SNARE complexes and utilizes the energy of ATP hydrolysis to disassemble them thus facilitating SNARE recycling. While this is a major function of NSF, it does seem to interact with other proteins, such as the AMPA receptor subunit, GluR2, and beta2-AR and is thought to affect their trafficking patterns.

Protein type: Autophagy; Vesicle; Adaptor/scaffold; Protease; EC 3.6.4.6

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: Golgi stack; lysosomal membrane; cytoplasm; postsynaptic density; plasma membrane; dendritic shaft; cytosol

Molecular Function: protein binding; metal ion binding; ATPase activity; syntaxin-1 binding; protein complex binding; ATPase activity, coupled; protein kinase binding; Rab GTPase binding; ATP binding; PDZ domain binding

Biological Process: regulation of exocytosis; vesicle-mediated transport; intra-Golgi vesicle-mediated transport; synaptic transmission; exocytosis; metabolic process; Golgi to plasma membrane protein transport; Golgi vesicle docking; positive regulation of receptor recycling; potassium ion transport; plasma membrane fusion