Rabbit anti-Human, rat NSE (Neuron specific enolase) Polyclonal Antibody | anti-ENO2 antibody

Anti-Neuron Specific Enolase (NSE)

Cat. Num. AAA502094

• Gene Names: ENO2; NSE; HEL-S-279
• Reactivity: Human, rat
• Applications: Western Blot, Immunofluorescence
• Purity: Neat Serum (Unpurified neat serum.)
• Synonyms: NSE (Neuron specific enolase); Polyclonal Antibody; Anti-Neuron Specific Enolase (NSE); anti-ENO2 antibody

• Host: Rabbit
• Reactivity: Human, rat
• Clonality: Polyclonal
• Specificity: Specific for the ~47kDa NSE protein.
• Purity/Purification: Neat Serum (Unpurified neat serum.)
• Form/Format: Liquid.
• Sequence Length: 434

• Applicable Applications for anti-ENO2 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:2,000; IF: 1:500
• Antigen: Recombinant human NSE expressed in and purified from E. coli.
• Immunogen Information: Recombinant human NSE expressed in and purified from E. coli
• Immunogen Species: Human
• Species Reactivity Note: The antibody has been directly tested for reactivity in human and rat. It is expected that it will work on other mammalian tissues.
• Biological Significance: Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers.(MacAlesse et al., 1988). NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury (2).
• Preparation and Storage: Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

Testing Data

Related Product Information for anti-ENO2 antibody

Rabbit polyclonal antibody

References

• MacAlesse SM, Dunbar B, Fotherqill JE, Hinks LJ, Day IN (1988) Complete amino acid sequence of neurone-specific gamma isozyme of enolase (NSE) from human brain and comparison with the non-neuronal alpha form (NNE). Eur J Biochem. 178(2):413-17.
• Begaz, T., Kyriacou, D. N., Segal, J. and Bazarian, J. J. Serum biochemical markers for post-concussion syndrome in patients with mild traumatic brain injury. J. Neurotrauma 23:1201-1210 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 5803011
• NCBI GeneID: 2026
• NCBI Accession #: NP_001966.1
• NCBI GenBank Nucleotide #: NM_001975.2
• UniProt Accession #: P09104; Q96J33
• Molecular Weight: 47
• NCBI Official Full Name: gamma-enolase
• NCBI Official Synonym Full Names: enolase 2 (gamma, neuronal)
• NCBI Official Symbol: ENO2
• NCBI Official Synonym Symbols: NSE; HEL-S-279
• NCBI Protein Information: gamma-enolase; neural enolase; neuron-specific enolase; neurone-specific enolase; neuron specific gamma enolase; 2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; epididymis secretory protein Li 279
• UniProt Protein Name: Gamma-enolase
• Protein Family: Enolase
• UniProt Gene Name: ENO2
• UniProt Synonym Gene Names: NSE
• UniProt Entry Name: ENOG_HUMAN

NCBI Description

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival

By similarity.

Catalytic activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactor: Magnesium. Required for catalysis and for stabilizing the dimer.

Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

Subunit structure: Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.

Subcellular location: Cytoplasm

By similarity. Cell membrane

By similarity. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form

By similarity.

Tissue specificity: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

Developmental stage: During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

Induction: Levels of ENO2 increase dramatically in cardiovascular accidents, cerebral trauma, brain tumors and Creutzfeldt-Jakob disease.

Sequence similarities: Belongs to the enolase family.

Sequence caution: The sequence AAA52388.1 differs from that shown. Reason: Erroneous initiation.