Rabbit anti-Human NSE Polyclonal Antibody | anti-NSE antibody

NSE (Y236) (Gamma-enolase, 2-phospho-D-glycerate Hydro-lyase, Neural Enolase, Neuron-specific Enolase, NSE, Enolase 2, ENO2, NSE) (AP)

Cat. Num. AAA6489901

• Gene Names: ENO2; NSE; HEL-S-279
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: NSE; Polyclonal Antibody; NSE (Y236) (Gamma-enolase; 2-phospho-D-glycerate Hydro-lyase; Neural Enolase; Neuron-specific Enolase; Enolase 2; ENO2; NSE) (AP); anti-NSE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NSE.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-NSE antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding Y236 of human NSE (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-NSE Antibody (Y236) in Y79 cell line lysates (35ug/lane). NSE (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(NSE Antibody (Y236) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NSE Antibody (Y236) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-NSE antibody

Antibodies; Neuron Specific Enolase

References

1. Kotaska, K., Neuro Endocrinol. Lett. 28(6), 761-764 (2007). 2. Forooghian, F., J. Clin. Immunol. 27(4), 388-396 (2007). 3. Rech, T.H., Crit Care 10(5), R133 (2006). 4. Oliva, D., Genomics 10(1), 157-165 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 5803011
• NCBI GeneID: 2026
• NCBI Accession #: NP_001966
• NCBI GenBank Nucleotide #: NM_001975.2
• UniProt Accession #: P09104; Q96J33; B7Z2X9
• Molecular Weight: 42,707 Da
• NCBI Official Full Name: gamma-enolase
• NCBI Official Synonym Full Names: enolase 2
• NCBI Official Symbol: ENO2
• NCBI Official Synonym Symbols: NSE; HEL-S-279
• NCBI Protein Information: gamma-enolase
• UniProt Protein Name: Gamma-enolase
• UniProt Gene Name: ENO2
• UniProt Synonym Gene Names: NSE
• UniProt Entry Name: ENOG_HUMAN

NCBI Description

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]

Uniprot Description

ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase; EC 4.2.1.11

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: extracellular space; photoreceptor inner segment; phosphopyruvate hydratase complex; plasma membrane; perikaryon; cytosol

Molecular Function: magnesium ion binding; phosphopyruvate hydratase activity

Biological Process: glycolysis; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis