Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using NSDHL antibody.)
Rabbit NSDHL Polyclonal Antibody | anti-NSDHL antibody
NSDHL Antibody
Gene Names
NSDHL; H105E3; XAP104; SDR31E1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NSDHL; Polyclonal Antibody; NSDHL Antibody; decarboxylating; H105E3; H105e3 protein; NAD(P) dependent steroid dehydrogenase like; NSDHL_HUMAN; Protein H105e3; SDR31E1; Short chain dehydrogenase/reductase family 31E member 1; Sterol 4 alpha carboxylate 3 dehydrogenase decarboxylating; Sterol-4-alpha-carboxylate 3-dehydrogenase; XAP104; anti-NSDHL antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NSDHL antibody detects endogenous levels of NSDHL.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
373
Applicable Applications for anti-NSDHL antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human NSDHL.
Subcellular Location
Endoplasmic Reticulum
Tissue Specificity
Brain, heart, liver, lung, kidney, skin and placenta.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Zebrafish (92%), Bovine (92%), Horse (83%), Sheep (92%), Rabbit (100%), Dog (100%), Chicken (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using NSDHL antibody.)
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using NSDHL antibody.)
Immunohistochemistry (IHC)
(MBS9603660 at 1/100 staining Human pancreas cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603660 at 1/100 staining Human pancreas cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-NSDHL antibody
Function: Involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis.
Similarity: Belongs to the 3-beta-HSD family.
Similarity: Belongs to the 3-beta-HSD family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 42 kDa; 40 kDa
Predicted: 42 kDa
Predicted: 42 kDa
NCBI Official Full Name
sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating
NCBI Official Synonym Full Names
NAD(P) dependent steroid dehydrogenase-like
NCBI Official Symbol
NSDHL
NCBI Official Synonym Symbols
H105E3; XAP104; SDR31E1
NCBI Protein Information
sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating
UniProt Protein Name
Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating
UniProt Gene Name
NSDHL
UniProt Synonym Gene Names
H105E3
NCBI Description
The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis.
Research Articles on NSDHL
Similar Products
Product Notes
The NSDHL nsdhl (Catalog #AAA9603660) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NSDHL Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NSDHL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the NSDHL nsdhl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NSDHL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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