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Western Blot (WB) (Western blot analysis of Nrf2 phosphorylation expression in HepG2 cell lysate.)

Rabbit anti-Human Nrf2 Polyclonal Antibody | anti-Nrf2 antibody

Anti-Phospho-Nrf2 (S40) Rabbit pAb

Gene Names
NFE2L2; NRF2; HEBP1; IMDDHH
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity Chromatography
Synonyms
Nrf2; Polyclonal Antibody; Anti-Phospho-Nrf2 (S40) Rabbit pAb; anti-Nrf2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-Nrf2 (S40) Antibody detects endogenous levels of total Phospho-Nrf2 (S40)
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
589
Applicable Applications for anti-Nrf2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:50
Immunogen
A synthesized peptide derived from human Phospho-Nrf2 (S40)
Subcellular Location
Cytoplasm
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot analysis of Nrf2 phosphorylation expression in HepG2 cell lysate.)

Western Blot (WB) (Western blot analysis of Nrf2 phosphorylation expression in HepG2 cell lysate.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Nrf2 (S40) Antibody.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Nrf2 (S40) Antibody.)

Immunofluorescence (IF)

(Immunofluorescent analysis of HepG2 cells, using Phospho-Nrf2 (S40) Antibody.)

Immunofluorescence (IF) (Immunofluorescent analysis of HepG2 cells, using Phospho-Nrf2 (S40) Antibody.)
Related Product Information for anti-Nrf2 antibody
Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
90kDa
NCBI Official Full Name
nuclear factor erythroid 2-related factor 2 isoform 2
NCBI Official Synonym Full Names
nuclear factor, erythroid 2 like 2
NCBI Official Symbol
NFE2L2
NCBI Official Synonym Symbols
NRF2; HEBP1; IMDDHH
NCBI Protein Information
nuclear factor erythroid 2-related factor 2
UniProt Protein Name
Nuclear factor erythroid 2-related factor 2
UniProt Gene Name
NFE2L2
UniProt Synonym Gene Names
NRF2; NF-E2-related factor 2; NFE2-related factor 2

NCBI Description

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

Research Articles on Nrf2

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Product Notes

The Nrf2 nfe2l2 (Catalog #AAA4750305) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Phospho-Nrf2 (S40) Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nrf2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC/IF: 1:50-1:200 IP: 1:50 FC/FACS: 1:50. Researchers should empirically determine the suitability of the Nrf2 nfe2l2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nrf2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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