Rabbit NRF1 Polyclonal Antibody | anti-NRF1 antibody

Anti-NRF1 antibody

Cat. Num. AAA176123

• Gene Names: NRF1; ALPHA-PAL
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen affinity purified.
• Synonyms: NRF1; Polyclonal Antibody; Anti-NRF1 antibody; Nuclear respiratory factor 1; nuclear respiratory factor 1; alpha pal antibody; alpha palindromic binding protein antibody; Alpha palindromic-binding protein antibody; Alpha-pal antibody; locus control region factor 1 antibody; NFE2 related factor 1 antibody; NRF-1 antibody; Nrf1 antibody; NRF1_HUMAN antibody; nuclear factor(erythroid derived 2)-like 1 antibody; nuclear respiratory factor 1 antibody; transcription factor 11 antibody; anti-NRF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 503

• Applicable Applications for anti-NRF1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human NRF1 (272-288aa QHGREDLLYAFEDQQTQ), identical to the related rat and mouse sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Testing Data

(All lanes: Anti NRF1 (MBS176123) at 0.5ug/mlLane 1: U2OS Whole Cell Lysate at 40ugLane 2: HELA Whole Cell Lysate at 40ugPredicted bind size: 75KDObserved bind size: 75KD )

Immunohistochemistry (IHC)

(Anti-NRF1 antibody, MBS176123, IHC(P)IHC(P): Human Mammary Cancer Tissue )

Immunocytochemistry (ICC)

(Anti-NRF1 antibody, MBS176123, ICCICC: A549 Cell )

Immunohistochemistry (IHC)

(Anti-NRF1 antibody, MBS176123, IHC(P)IHC(P): Rat Intestine Tissue )

Immunohistochemistry (IHC)

(Anti-NRF1 antibody, MBS176123, IHC(P)IHC(P): Human Intestinal Cance Tissue )

Related Product Information for anti-NRF1 antibody

Description: Rabbit IgG polyclonal antibody for Nuclear respiratory factor 1(NRF1) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: NRF1(Nuclear Respiratory Factor 1), also known as Alpha-Pal. Gopalakrishnan and Scarpulla(1995) analyzed DNA from a panel of human/hamster cell hybrids using human-specific NRF1 PCR primers and localized the NRF1 gene to human chromosome 7. The assignment was further refined to 7q31 by cohybridization of NRF1- and chromosome 7-specific probes to human metaphase chromosomes. Efiok et al.(1994) identified genes containing alpha-Pal-binding sequences and found that these could be classified either as cellular proliferation genes, or as genes regulating the growth-responsive metabolic pathways of energy transduction, translation, and replication. Virbasius and Scarpulla(1994) noted that the nuclear-encoded mitochondrial transcription factor TFAM contains potential binding sites for NRF1, NRF2(GABPA) and SP1 within the promoter region. With use of binding and electrophoretic mobility shift assays, DNase footprinting, and mutation analysis of recombinant proteins, they demonstrated specific and functional binding of NRF1 and NRF2 to the TFAM promoter region.

NCBI and Uniprot Product Information

• NCBI GI #: 12643732
• NCBI GeneID: 4899
• UniProt Accession #: Q16656; Q15305; Q96AN2; A8K4C6; B4DDV6
• Molecular Weight: 55,674 Da
• NCBI Official Full Name: Nuclear respiratory factor 1
• NCBI Official Synonym Full Names: nuclear respiratory factor 1
• NCBI Official Symbol: NRF1
• NCBI Official Synonym Symbols: ALPHA-PAL
• NCBI Protein Information: nuclear respiratory factor 1; alpha palindromic-binding protein
• UniProt Protein Name: Nuclear respiratory factor 1
• Protein Family: Nuclear respiratory factor
• UniProt Gene Name: NRF1
• UniProt Synonym Gene Names: NRF-1; Alpha-pal
• UniProt Entry Name: NRF1_HUMAN

NCBI Description

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014]

Uniprot Description

NRF1: Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. Belongs to the NRF1/Ewg family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: nucleoplasm; cytoplasm

Biological Process: regulation of transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; generation of precursor metabolites and energy; organ regeneration; transcription, DNA-dependent; response to folic acid; organelle organization and biogenesis; response to hypoxia; response to lipopolysaccharide; positive regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; response to electrical stimulus; response to estradiol stimulus