Rabbit anti-Human, Mouse NRBP Polyclonal Antibody | anti-NRBP1 antibody

NRBP, NT (Nuclear Receptor Binding Protein, Nrbp1, BCON3, FLJ27109, FLJ35541, HLS7-interacting Protein Kinase, Multiple Domain Putative Nuclear Protein, MUDPNP, Myeloid Leukemia Factor 1 Adaptor Molecule, MLF1 Adapter Molecule, MADM)

Cat. Num. AAA624903

• Gene Names: NRBP1; MADM; NRBP; BCON3; MUDPNP; FLJ27109; FLJ35541
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: NRBP; Polyclonal Antibody; NT (Nuclear Receptor Binding Protein; Nrbp1; BCON3; FLJ27109; FLJ35541; HLS7-interacting Protein Kinase; Multiple Domain Putative Nuclear Protein; MUDPNP; Myeloid Leukemia Factor 1 Adaptor Molecule; MLF1 Adapter Molecule; MADM); Anti -NRBP; anti-NRBP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NRBP. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-NRBP1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human NRBP (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-NRBP Pab in HL-60 cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2). NRBP (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-NRBP1 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Product Categories/Family for anti-NRBP1 antibody

Antibodies; Abs to Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 7019333
• NCBI GeneID: 29959
• NCBI Accession #: NP_037524.1
• NCBI GenBank Nucleotide #: NM_013392.2
• UniProt Accession #: Q9UHY1; Q53FZ5; Q96SU3; B3KV40; D6W558
• Molecular Weight: 59,845 Da
• NCBI Official Full Name: nuclear receptor-binding protein
• NCBI Official Synonym Full Names: nuclear receptor binding protein 1
• NCBI Official Symbol: NRBP1
• NCBI Official Synonym Symbols: MADM; NRBP; BCON3; MUDPNP; FLJ27109; FLJ35541
• NCBI Protein Information: nuclear receptor-binding protein; OTTHUMP00000123427; OTTHUMP00000123428; OTTHUMP00000200794; multiple domain putative nuclear protein; myeloid leukemia factor 1 adaptor molecule
• UniProt Protein Name: Nuclear receptor-binding protein
• Protein Family: Nuclear receptor-binding protein
• UniProt Gene Name: NRBP1
• UniProt Synonym Gene Names: BCON3; NRBP
• UniProt Entry Name: NRBP_HUMAN

Uniprot Description

NRBP1: May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; Other group; NRBP family

Chromosomal Location of Human Ortholog: 2p23

Cellular Component: nucleoplasm; membrane; perinuclear region of cytoplasm; lamellipodium; endomembrane system; cell cortex

Molecular Function: protein binding; protein homodimerization activity; ATP binding; protein kinase activity

Biological Process: transcription initiation from RNA polymerase II promoter; ER to Golgi vesicle-mediated transport; gene expression; protein amino acid phosphorylation