Rabbit NR6A1 / GCNF Polyclonal Antibody | anti-NR6A1 antibody

Rabbit Polyclonal to Human NR6A1 / GCNF

Cat. Num. AAA246731

• Gene Names: NR6A1; RTR; GCNF; NR61; hRTR; CT150; GCNF1; hGCNF
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: NR6A1 / GCNF; Polyclonal Antibody; Rabbit Polyclonal to Human NR6A1 / GCNF; NR6A1 Antibody; Germ cell nuclear factor Antibody; HGCNF Antibody; NR61 Antibody; HRTR Antibody; RTR Antibody; Ncnf Antibody; GCNF Antibody; GCNF1 Antibody; anti-NR6A1 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
• Clonality: Polyclonal
• Specificity: Human NR6A1 / GCNF. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 480

• Applicable Applications for anti-NR6A1 antibody: IHC - Paraffin
• Application Notes: IHC-P (10 ug/ml); Optimal dilution to be determined by the researcher.
• Target: NR6A1/GCNF
• Target Species: Nuclear receptor subfamily 6, group A, member 1 (NR6A1)
• Immunogen Description: Synthetic 18 amino acid peptide from ligand-binding domain of human NR6A1 / GCNF.
• Family/Subfamily: NHR/ NR6 Germ cell nuclear factor-like)
• Immunogen Species: NR6A1/GCNF antibody was rasied agianst Human.
• Immunogen Type: Synthetic peptide
• Immunogen: NR6A1 / GCNF antibody was raised against synthetic 18 amino acid peptide from ligand-binding domain of human NR6A1/GCNF. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (100%); Platypus (89%); Turkey, Chicken (83%).
• Epitope: Ligand-binding Domain
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Upon arrival: Aliquot and store undilute at -20 degree C or below for up to 1 year. can be stored undilted at 4 degree C for up to 1 month. Aovid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-NR6A1 / GCNF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-NR6A1 antibody

Germ cell nuclear factor (GCNF), a NR6 Germ Cell Nuclear Factor-Like Receptor, has been shown to affect gene expression in embryogenesis and spermatogenesis and is essential for embryonic survival and normal development. Loss of GCNF causes embryonic lethality and disrupts normal somitogenesis as well as neural-tube and axis formation. GCNF binds as a homodimer to the DR0 DNA response element and has been shown to regulate transcription of genes including protamines1 and 2 and Oct4. GCNF's repression function has been shown to be mediated by interaction with the co-repressors N-COR and SMRT in the absence of ligand. Three protein isoforms, GCNF-1, GCNF-2a, and GCNF-2b, have been documented in humans, and they are generated by differential usage of alternative splice acceptor sites of the fourth and seventh exons. GCNF-1, the longest isoform, has a distinct 5' UTR and contains 4 and 26 additional amino acids than the other two forms, respectively.

Product Categories/Family for anti-NR6A1 antibody

Family: NHR; Subfamily: NR6 Germ cell nuclear factor-like

NCBI and Uniprot Product Information

• NCBI GI #: 53729325
• NCBI GeneID: 2649
• NCBI Accession #: NP_201591.2
• NCBI GenBank Nucleotide #: NM_033334.3
• UniProt Accession #: Q15406; O00551; O00603; Q5T6F4; Q8NHQ0; Q92898; Q99802
• Molecular Weight: 53,908 Da
• NCBI Official Full Name: nuclear receptor subfamily 6 group A member 1 isoform 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 6, group A, member 1
• NCBI Official Symbol: NR6A1
• NCBI Official Synonym Symbols: RTR; GCNF; NR61; hRTR; CT150; GCNF1; hGCNF
• NCBI Protein Information: nuclear receptor subfamily 6 group A member 1; germ cell nuclear factor variant 1; retinoid receptor-related testis-specific receptor; retinoic acid receptor-related testis-associated receptor
• UniProt Protein Name: Nuclear receptor subfamily 6 group A member 1
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR6A1
• UniProt Synonym Gene Names: GCNF; GCNF; hGCNF; RTR; hRTR
• UniProt Entry Name: NR6A1_HUMAN

NCBI Description

This gene encodes an orphan nuclear receptor which is a member of the nuclear hormone receptor family. Its expression pattern suggests that it may be involved in neurogenesis and germ cell development. The protein can homodimerize and bind DNA, but in vivo targets have not been identified. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2013]

Uniprot Description

NR6A1: Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis. Homodimer. Interacts with UIMC1. Shows highest expression in the germ cells of the adult testis. Belongs to the nuclear hormone receptor family. NR6 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: nucleoplasm; transcription factor complex

Molecular Function: ligand-dependent nuclear receptor activity; protein homodimerization activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity

Biological Process: cell proliferation; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; gamete generation; positive regulation of transcription from RNA polymerase II promoter; gene expression; spermatogenesis; steroid hormone mediated signaling; negative regulation of transcription from RNA polymerase II promoter