Rabbit anti-Human, Mouse NR6A1 Polyclonal Antibody | anti-NR6A1 antibody

NR6A1, NT (NR6A1, GCNF, Nuclear receptor subfamily 6 group A member 1, Germ cell nuclear factor, Retinoid receptor-related testis-specific receptor) (APC)

Cat. Num. AAA6326700

• Gene Names: NR6A1; RTR; GCNF; NR61; hRTR; CT150; GCNF1; hGCNF
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NR6A1; Polyclonal Antibody; NT (NR6A1; GCNF; Nuclear receptor subfamily 6 group A member 1; Germ cell nuclear factor; Retinoid receptor-related testis-specific receptor) (APC); Retinoid receptor-related testis-specific receptor; anti-NR6A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 475

• Applicable Applications for anti-NR6A1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NR6A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-67 amino acids from the N-terminal region of human NR6A1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NR6A1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NR6A1 antibody detected the NR6A1 protein (arrow).)

Western Blot (WB)

(NR6A1 Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the NR6A1 antibody detected the NR6A1 protein (arrow).)

Related Product Information for anti-NR6A1 antibody

This gene encodes an orphan nuclear receptor which is a member of the nuclear hormone receptor family. Its expression pattern suggests that it may be involved in neurogenesis and germ cell development. The protein can homodimerize and bind DNA, but in vivo targets have not been identified. The gene expresses at least alternatively spliced transcript variants.

Product Categories/Family for anti-NR6A1 antibody

Antibodies; Receptors

References

Letra, A., et al. Am. J. Med. Genet. A 152A (7), 1701-1710 (2010): Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Rajkovic, M., et al. J. Biol. Chem. 279(50):52493-52499(2004). Yang, G., et al. Biol. Reprod. 68(5):1620-1630(2003). Lan, Z.J., et al. J. Biol. Chem. 277(52):50660-50667(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 53729327
• NCBI GeneID: 2649
• NCBI Accession #: NP_001480.3; NP_201591.2
• NCBI GenBank Nucleotide #: NP_001480.3
• UniProt Accession #: Q15406
• NCBI Official Full Name: nuclear receptor subfamily 6 group A member 1 isoform 2
• NCBI Official Synonym Full Names: nuclear receptor subfamily 6 group A member 1
• NCBI Official Symbol: NR6A1
• NCBI Official Synonym Symbols: RTR; GCNF; NR61; hRTR; CT150; GCNF1; hGCNF
• NCBI Protein Information: nuclear receptor subfamily 6 group A member 1
• UniProt Protein Name: Nuclear receptor subfamily 6 group A member 1
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR6A1
• UniProt Synonym Gene Names: GCNF; GCNF; hGCNF; RTR; hRTR
• UniProt Entry Name: NR6A1_HUMAN

NCBI Description

This gene encodes an orphan nuclear receptor which is a member of the nuclear hormone receptor family. Its expression pattern suggests that it may be involved in neurogenesis and germ cell development. The protein can homodimerize and bind DNA, but in vivo targets have not been identified. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2013]

Uniprot Description

NR6A1: Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis. Homodimer. Interacts with UIMC1. Shows highest expression in the germ cells of the adult testis. Belongs to the nuclear hormone receptor family. NR6 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: nucleoplasm; transcription factor complex

Molecular Function: ligand-dependent nuclear receptor activity; protein homodimerization activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity

Biological Process: transcription initiation from RNA polymerase II promoter; cell proliferation; intracellular receptor-mediated signaling pathway; gamete generation; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; spermatogenesis; negative regulation of transcription from RNA polymerase II promoter