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Rabbit anti-Mouse NR2B Polyclonal Antibody | anti-NR2B antibody

NR2B (HRP)

Gene Names
GRIN2B; MRD6; NR2B; hNR3; GluN2B; NMDAR2B
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
NR2B; Polyclonal Antibody; NR2B (HRP); anti-NR2B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse NR2B at ~180kD. Species sequence homology: Sequence is identical to and may react with aa1437-1456 of mature rat NR2B and aa1465-1484 of human NR3 (containing a N-terminal lysine).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-NR2B antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 0.5-2ug/ml (~180kD). Detects NR2B in rat brain microsomal protein preparation.
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to aa1437-1456 of mature mouse NR2B or aa1463-1482 of NR2B precursor (NM_008171). Signal is aa1-26 (KFNGSSNGHV-YEKLSSIESDV). Species sequence homology: rat, human, bovine, canine and chimp.
Conjugate
HRP
Control
Mouse Brain Tissue
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Product Categories/Family for anti-NR2B antibody
References
1. Bliss, T.V.P., Collingridge, G.L., Nature 361: 31-39 (1993). 2. Choi, D.W., Rothman, S.M., Ann. Rev. Neurosci. 13: 171-182 (1990). 3. Constantine-Patton, M., et al., Ann. Rev. Nerosci. 13: 129-154 (1990). 4. Mandich, P., et al., Genomics 22: 216-218 (1994). 5. Sheng, M., et al., Nature 368: 144-147 (1994). 6. Rao, A., et al., J. Neurosci. 18: 1217-1229 (1998). 7. Rao, A., Craig, A.M., Neuron 19: 801-812 (1997). 8. Ehlers, M.D., et al., Cell 84: 745-755 (1996).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
166,367 Da
NCBI Official Full Name
glutamate receptor ionotropic, NMDA 2B
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, N-methyl D-aspartate 2B
NCBI Official Symbol
GRIN2B
NCBI Official Synonym Symbols
MRD6; NR2B; hNR3; GluN2B; NMDAR2B
NCBI Protein Information
glutamate receptor ionotropic, NMDA 2B; NR3; glutamate receptor subunit epsilon-2; N-methyl-D-aspartate receptor subunit 3; N-methyl D-aspartate receptor subtype 2B; glutamate [NMDA] receptor subunit epsilon-2
UniProt Protein Name
Glutamate receptor ionotropic, NMDA 2B
UniProt Gene Name
GRIN2B
UniProt Synonym Gene Names
NMDAR2B; GluN2B; NMDAR2B; NR2B; NR3; hNR3
UniProt Entry Name
NMDE2_HUMAN

NCBI Description

N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. [provided by RefSeq, Jul 2008]

Uniprot Description

NMDAR2B: an NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death.

Protein type: Channel, calcium; Membrane protein, integral; Channel, ligand-gated; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12p12

Cellular Component: postsynaptic membrane; synaptic vesicle; neuron projection; cell surface; integral to plasma membrane; dendrite; plasma membrane; cell junction; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; zinc ion binding; glycine binding; calcium channel activity; N-methyl-D-aspartate selective glutamate receptor activity

Biological Process: axon guidance; synaptic transmission, glutamatergic; behavioral fear response; startle response; in utero embryonic development; glutamate signaling pathway; regulation of synaptic plasticity; learning; memory; detection of mechanical stimulus involved in sensory perception of pain; synaptic transmission; behavioral response to pain; response to ethanol; sensory organ development; learning and/or memory; suckling behavior; transport; ionotropic glutamate receptor signaling pathway; ephrin receptor signaling pathway; regulation of excitatory postsynaptic membrane potential

Disease: Epileptic Encephalopathy, Early Infantile, 27; Mental Retardation, Autosomal Dominant 6

Research Articles on NR2B

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Product Notes

The NR2B grin2b (Catalog #AAA6490500) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NR2B (HRP) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NR2B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 0.5-2ug/ml (~180kD). Detects NR2B in rat brain microsomal protein preparation. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NR2B grin2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NR2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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