Rabbit anti-Human NR1H2 Polyclonal Antibody | anti-NR1H2 antibody

NR1H2, NT (Oxysterols Receptor LXR-beta, Liver X Receptor beta, Nuclear Receptor NER, Nuclear Receptor Subfamily 1 Group H Member 2, Ubiquitously-expressed Nuclear Receptor, LXRB, NER, UNR)

Cat. Num. AAA6008459

• Gene Names: NR1H2; NER; UNR; LXRB; LXR-b; NER-I; RIP15
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: NR1H2; Polyclonal Antibody; NT (Oxysterols Receptor LXR-beta; Liver X Receptor beta; Nuclear Receptor NER; Nuclear Receptor Subfamily 1 Group H Member 2; Ubiquitously-expressed Nuclear Receptor; LXRB; NER; UNR); Anti -NR1H2; anti-NR1H2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human LXRbeta. Species sequence homology: Mouse (85%), Rat (70%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

• Applicable Applications for anti-NR1H2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1mg/ml; Immunohistochemistry: 1:300
• Immunogen: Synthetic linear peptide corresponding to the N-terminus of human LXRbeta (KLH).
• Positive Control: Human cerebellum tissue lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-NR1H2 antibody

LXRa and LXRb (NR1H3 and NR1H2, respectively) are ligand-activated nuclear receptors that mediate cholesterol homeostasis. Once activated, LXRs upregulate the transcription of genes involved in cholesterol transport such as those encoding ATP-binding cassette (ABC) transporters, including ATP-binding cassette protein A (ABCA)1 and apolipoprotein E (Apo E), in various brain diseases. ABCA1 is known to carry and deliver cholesterol through receptor-mediated endocytosis, while in Alzheimer's brain, both ABCA1 and Apo E are shown to influence amyloid-beta transport and clearance. It is also shown that LXR ligands inhibit STAT1 binding to related gene promoters and the downstream expression of IRF1, TNFa, and IL-6. One study reports that differential SUMOlyation of LXRa and LXRb positively transrepresses STAT1 inflammatory signaling in IFN-g-stimulated rat brain astrocytes and that transient tranfection of specific individual siRNAs against LXRb and LXRa blocks LXR-induced suppression of IRF1 and TNFa expression.

Product Categories/Family for anti-NR1H2 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 48145529
• NCBI GeneID: 7376
• UniProt Accession #: Q6IBU6; P55055
• Molecular Weight: 51,088 Da
• NCBI Official Full Name: NR1H2
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1, group H, member 2
• NCBI Official Symbol: NR1H2
• NCBI Official Synonym Symbols: NER; UNR; LXRB; LXR-b; NER-I; RIP15
• NCBI Protein Information: oxysterols receptor LXR-beta; LX receptor beta; nuclear receptor NER; liver X nuclear receptor beta; nuclear orphan receptor LXR-beta; steroid hormone-nuclear receptor NER; ubiquitously-expressed nuclear receptor
• UniProt Protein Name: NR1H2 protein
• Protein Family: Oxysterols receptor
• UniProt Gene Name: NR1H2
• UniProt Entry Name: Q6IBU6_HUMAN

NCBI Description

The liver X receptors, LXRA (NR1H3; MIM 602423) and LXRB, form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver, adrenal gland, intestine, adipose tissue, macrophages, lung, and kidney, whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors (RXRs; see MIM 180245) and regulate expression of target genes containing LXR response elements (summary by Korf et al., 2009 [PubMed 19436111]).[supplied by OMIM, Jan 2010]

Uniprot Description

LXR-beta: Orphan receptor. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Forms a heterodimer with RXR. Ubiquitous. Belongs to the nuclear hormone receptor family. NR1 subfamily.

Protein type: Nuclear receptor

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: retinoid X receptor binding; ligand-dependent nuclear receptor activity; protein binding; DNA binding; zinc ion binding; steroid hormone receptor activity; apolipoprotein A-I receptor binding; ATPase binding

Biological Process: retinoic acid receptor signaling pathway; negative regulation of lipid transport; negative regulation of proteolysis; transcription initiation from RNA polymerase II promoter; positive regulation of fatty acid biosynthetic process; positive regulation of cellular protein metabolic process; negative regulation of pinocytosis; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cholesterol transport; cholesterol homeostasis; positive regulation of lipoprotein lipase activity; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; negative regulation of transcription, DNA-dependent