Rabbit NR1D1 Polyclonal Antibody | anti-NR1D1 antibody

Rabbit Polyclonal to Human NR1D1

Cat. Num. AAA243226

• Gene Names: NR1D1; EAR1; hRev; THRA1; THRAL; ear-1
• Reactivity: Gibbon, Gorilla, Human, Monkey; Predicted Reactivity: Mouse, Rat, Bovine, Dog, Hamster, Horse, Rabbit (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: NR1D1; Polyclonal Antibody; Rabbit Polyclonal to Human NR1D1; Anti-NR1D1 Antibody (Internal) IHC-plus; EAR1; Rev-erbA-alpha; Reverba; Reverbaalpha; Rev-erb alpha; Rev-erba; Rev-erbalpha; RevErbAlpha; THRA1; Rev-ErbAalpha; Ear-1; HRev; THRAL; V-erbA-related protein 1; Human NR1D1; anti-NR1D1 antibody

• Host: Rabbit
• Reactivity: Gibbon, Gorilla, Human, Monkey; Predicted Reactivity: Mouse, Rat, Bovine, Dog, Hamster, Horse, Rabbit (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human NR1D1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 614

• Applicable Applications for anti-NR1D1 antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (6 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic 18 amino acid peptide from internal region of human NR1D1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Mouse, Rat, Hamster, Panda, Dog, Bovine, Horse, Rabbit (94%).
• Immunogen Type: Synthetic peptide
• Immunogen: NR1D1 antibody was raised against synthetic 18 amino acid peptide from internal region of human NR1D1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Mouse, Rat, Hamster, Panda, Dog, Bovine, Horse, Rabbit (94%); Elephant, Bat, Opossum (89%).
• Antigen Modification: Internal
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-NR1D1 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-NR1D1 antibody

Rev-erbA alpha, a NR1 Thyroid Hormone-Like Receptor, is encoded by the same genomic locus as, but transcribed from the opposite strand of, Thyroid Hormone Receptor Alpha (TR Alpha). Rev-erbA alpha is a target of Nuclear Receptor ROR Alpha (Delerive et al. 2002), and a transcription regulator that has been shown to affect myocyte differentiation, adipogenesis, and lipoprotein metabolism.

Product Categories/Family for anti-NR1D1 antibody

Family: NHR; Subfamily: NR1 Thyroid hormone-like

NCBI and Uniprot Product Information

• NCBI GI #: 13430848
• NCBI GeneID: 9572
• NCBI Accession #: NP_068370.1
• NCBI GenBank Nucleotide #: NM_021724.4
• UniProt Accession #: P20393; Q0P5Z4; Q15304
• Molecular Weight: 66,805 Da
• NCBI Official Full Name: nuclear receptor subfamily 1 group D member 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1, group D, member 1
• NCBI Official Symbol: NR1D1
• NCBI Official Synonym Symbols: EAR1; hRev; THRA1; THRAL; ear-1
• NCBI Protein Information: nuclear receptor subfamily 1 group D member 1; Rev-ErbAalpha; rev-erbA-alpha; V-erbA-related protein 1; nuclear receptor Rev-ErbA-alpha
• UniProt Protein Name: Nuclear receptor subfamily 1 group D member 1
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR1D1
• UniProt Synonym Gene Names: EAR1; HREV; THRAL; EAR-1
• UniProt Entry Name: NR1D1_HUMAN

NCBI Description

This gene encodes a transcription factor that is a member of the nuclear receptor subfamily 1. The encoded protein is a ligand-sensitive transcription factor that negatively regulates the expression of core clock proteins. In particular this protein represses the circadian clock transcription factor aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL). This protein may also be involved in regulating genes that function in metabolic, inflammatory and cardiovascular processes. [provided by RefSeq, Jan 2013]

Uniprot Description

NR1D1: a widely expressed member of the orphan nuclear receptor family of proteins. Expressed at relatively high levels in adipose tissue, skeletal muscle, brain and liver, and regulates cellular proliferation and differentiation. Expression increases during differentiation in adipocytes and its ectopic expression in 3T3-L1 cells potentiates adipocyte differentiation. A metabolic regulator whose expression is increased in HER2-positive breast cancer cells. Regulates malate dehydrogenase 1 (MDH1) and malic enzyme 1 (ME1), enzymes that link glycolysis and fatty acid synthesis. May contribute to the abnormal cellular energy metabolism observed in HER2-positive breast cancers. Its expression oscillates with circadian rhythm in liver cells, regulating the expression of BMAL1, ApoA1 and ApoC3, all key regulators of circadian rhythm. Represses the basal activity of the mouse Bmal1 gene promoter. Phosphorylation by GSK3b at S55/59 stabilizes NRD1 protein levels. Regulates inflammation by targeting the NF-??B responsive genes IL-6 and COX-2. NR1D1 lacks the activation function 2 domain required for ligand-dependent activation of transcription by other members of the nuclear receptor family; thus it behaves as a constitutive repressor protein, recruiting the nuclear receptor co-repressor N-CoR1/HDAC3 complex to target genes to repress transcription.

Protein type: Transcription factor; Nuclear receptor

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; nuclear chromatin; cytoplasm; dendrite; dendritic spine; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; zinc ion binding; heme binding; steroid hormone receptor activity; transcription corepressor activity

Biological Process: proteasomal protein catabolic process; circadian rhythm; circadian thermoregulation; transcription initiation from RNA polymerase II promoter; glycogen biosynthetic process; regulation of lipid metabolic process; intracellular receptor-mediated signaling pathway; regulation of fat cell differentiation; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; regulation of circadian rhythm; negative regulation of toll-like receptor 4 signaling pathway; gene expression; steroid hormone mediated signaling; circadian regulation of gene expression; cell differentiation; negative regulation of transcription, DNA-dependent