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Immunohistochemistry (IHC) (Anti-NQO1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Goat NQO1 Polyclonal Antibody | anti-NQO1 antibody

Anti-NQO1 Antibody (aa263-274)

Gene Names
NQO1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI
Reactivity
Human
Predicted: Rabbit, Mouse, Dog, Monkey
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
NQO1; Polyclonal Antibody; Anti-NQO1 Antibody (aa263-274); Mouse Monoclonal [clone A180] (IgG1) to Human NQO1; Azoreductase; DHQU; DT-diaphorase; Dioxin-inducible 1; DIA4; Diaphorase-4; NAD(P)H:quinone oxireductase; Phylloquinone reductase; QR1; Menadione reductase; NMOR1; NMORI; DTD; Quinone reductase 1; anti-NQO1 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Human
Predicted: Rabbit, Mouse, Dog, Monkey
Clonality
Polyclonal
Specificity
Human NQO1. This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1).
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Sequence Length
274
Applicable Applications for anti-NQO1 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 5 ug/ml
WB: 0.03-0.1 ug/ml
Immunogen
Synthetic peptide C-SIPTDNQIKARK from the C-terminus of human NQO1 (NP_000894.1; NP_001020604.1; NP_001020605.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Panda, Dog, Rabbit (100%); Mouse, Rat, Elephant, Bat, Horse, Pig, Guinea pig (92%); Hamster, Bovine, Opossum, Platypus (83%).
Conjugation
Unconjugated
Epitope
aa263-274
Preparation and Storage
Store at -20 degree C.
Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-NQO1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC) (Anti-NQO1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b)

Immunofluorescence (IF) (Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b)

Western Blot (WB)

(Staining (0.03?g/ml) of Human Kidney lysate (RIPA buffer, 35?g total protein per lane). Detected by chemiluminescence.)

Western Blot (WB) (Staining (0.03?g/ml) of Human Kidney lysate (RIPA buffer, 35?g total protein per lane). Detected by chemiluminescence.)

Western Blot (WB)

(EB05370 (1ug/ml) staining of Rat (A) and Pig (B) Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)

Western Blot (WB) (EB05370 (1ug/ml) staining of Rat (A) and Pig (B) Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)

Western Blot (WB)

(Antibody staining (1 ug/ml) of Human Lung lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)

Western Blot (WB) (Antibody staining (1 ug/ml) of Human Lung lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)

Western Blot (WB)

(EB05370 staining (0.03ug/ml) of Human Kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

Western Blot (WB) (EB05370 staining (0.03ug/ml) of Human Kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Related Product Information for anti-NQO1 antibody
is a member of the NAD(P)H dehydrogenase (quinone) family and encodes acytoplasmic 2-electron reductase. This FAD-binding protein formshomodimers and reduces quinones to hydroquinones. This protein 'senzymatic activity prevents the one electron reduction of quinones thatresults in the production of radical species.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26,365 Da
NCBI Official Full Name
NAD(P)H dehydrogenase
NCBI Official Synonym Full Names
NAD(P)H quinone dehydrogenase 1
NCBI Official Symbol
NQO1
NCBI Official Synonym Symbols
DTD; QR1; DHQU; DIA4; NMOR1; NMORI
NCBI Protein Information
NAD(P)H dehydrogenase [quinone] 1
UniProt Protein Name
NAD(P)H dehydrogenase [quinone] 1
Protein Family
UniProt Gene Name
NQO1
UniProt Synonym Gene Names
DIA4; NMOR1; DTD; QR1

NCBI Description

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.

Research Articles on NQO1

Similar Products

Product Notes

The NQO1 nqo1 (Catalog #AAA2401016) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Anti-NQO1 Antibody (aa263-274) reacts with Human Predicted: Rabbit, Mouse, Dog, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's NQO1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 5 ug/ml WB: 0.03-0.1 ug/ml. Researchers should empirically determine the suitability of the NQO1 nqo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NQO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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