Rabbit anti-Human NPTX1 Polyclonal Antibody | anti-NPTX1 antibody

NPTX1, CT (NP1, Neuronal Pentraxin I, NP-I, 47kD Taipoxin-binding Protein) (FITC)

Cat. Num. AAA6490410

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: NPTX1; Polyclonal Antibody; CT (NP1; Neuronal Pentraxin I; NP-I; 47kD Taipoxin-binding Protein) (FITC); anti-NPTX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NP1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NPTX1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human NP1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Nptx1 Antibody (C-term) in 293 cell line lysates (35ug/lane). Nptx1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with Nptx1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-NPTX1 antibody

Antibodies; Receptors

References

1. Hossain, M.A., J. Neurosci. 24(17), 4187-4196 (2004). 2. Schlimgen, A.K., Neuron 14(3), 519-526 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 24308510
• NCBI GeneID: 266777
• NCBI Accession #: NP_714957
• NCBI GenBank Nucleotide #: NM_153735.2
• UniProt Accession #: P47971; Q62443
• Molecular Weight: 47,216 Da
• NCBI Official Full Name: neuronal pentraxin-1
• NCBI Official Synonym Full Names: neuronal pentraxin 1
• NCBI Official Symbol: Nptx1
• NCBI Protein Information: neuronal pentraxin-1
• UniProt Protein Name: Neuronal pentraxin-1
• Protein Family: Neuronal pentraxin
• UniProt Gene Name: Nptx1
• UniProt Synonym Gene Names: NP1; NP-I

NCBI Description

secreted protein; may be involved in uptake of synaptic macromolecules [RGD, Feb 2006]

Uniprot Description

May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses.