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Western Blot (WB) ((0.3ug/ml) staining of Human (A) and Mouse (B) Kidney lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat NPHS2/SRN1 Polyclonal Antibody | anti-NPHS2 antibody

Goat anti-NPHS2/SRN1 Antibody

Gene Names
NPHS2; PDCN; SRN1
Reactivity
Tested: Human, Mouse, Pig; Expected from sequence similarity: Human, Mouse, Pig, Cow,
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
NPHS2/SRN1; Polyclonal Antibody; Goat anti-NPHS2/SRN1 Antibody; NPHS2; nephrosis 2; idiopathic; steroid-resistant (podocin); PDCN; SRN1; podocin; NPHS2 antibody; steroid-resistant (podocin) antibody; PDCN antibody; SRN1 antibody; podocin antibody; NPHS2 / SRN1; anti-NPHS2 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human, Mouse, Pig; Expected from sequence similarity: Human, Mouse, Pig, Cow,
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
SPSKPVEPLNPKK
Sequence Length
383
Applicable Applications for anti-NPHS2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 45kDa band observed in Human, Mouse and Pig Kidney lysates (calculated MW of 42.2kDa according to NP_055440.1). Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-SPSKPVEPLNPKK, from the C Terminus of the protein sequence according to NP_055440.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of Human (A) and Mouse (B) Kidney lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.3ug/ml) staining of Human (A) and Mouse (B) Kidney lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

((1ug/ml) staining of Pig Kidney lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((1ug/ml) staining of Pig Kidney lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,421 Da
NCBI Official Full Name
podocin isoform 1
NCBI Official Synonym Full Names
NPHS2 podocin
NCBI Official Symbol
NPHS2
NCBI Official Synonym Symbols
PDCN; SRN1
NCBI Protein Information
podocin
UniProt Protein Name
Podocin
Protein Family
UniProt Gene Name
NPHS2
UniProt Entry Name
PODO_HUMAN

NCBI Description

This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

NPHS2: Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2). It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. Belongs to the band 7/mec-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: endoplasmic reticulum; integral to plasma membrane; intercellular junction; lipid raft; plasma membrane; protein complex

Molecular Function: protein binding

Biological Process: actin cytoskeleton reorganization; excretion

Disease: Nephrotic Syndrome, Type 2

Research Articles on NPHS2

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Product Notes

The NPHS2 nphs2 (Catalog #AAA423330) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-NPHS2/SRN1 Antibody reacts with Tested: Human, Mouse, Pig; Expected from sequence similarity: Human, Mouse, Pig, Cow, and may cross-react with other species as described in the data sheet. AAA Biotech's NPHS2/SRN1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 45kDa band observed in Human, Mouse and Pig Kidney lysates (calculated MW of 42.2kDa according to NP_055440.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the NPHS2 nphs2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SPSKPVEPLN PKK. It is sometimes possible for the material contained within the vial of "NPHS2/SRN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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