Rabbit anti-Human NPHP4 Polyclonal Antibody | anti-NPHP4 antibody

NPHP4 Antibody

Cat. Num. AAA9506585

• Gene Names: NPHP4; POC10; SLSN4
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Synonyms: NPHP4; Polyclonal Antibody; NPHP4 Antibody; Nephrocystin-4; Nephroretinin; anti-NPHP4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: NPHP4 Antibody detects endogenous levels of total NPHP4 protein.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 911

• Applicable Applications for anti-NPHP4 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50~1:100; ELISA: 1:40000
• Target Modification: Unmodified/Total
• Immunogen: The antiserum was produced against synthesized peptide derived from human NPHP4.
• Immunogen Range: 877-926
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain, using NPHP4 Antibody. The picture on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 615276301
• NCBI GeneID: 261734
• NCBI Accession #: NP_001278522.1
• NCBI GenBank Nucleotide #: NM_001291593.1
• UniProt Accession #: O75161; Q8IWC0
• Molecular Weight: 157 kDa
• NCBI Official Full Name: nephrocystin-4 isoform b
• NCBI Official Synonym Full Names: nephrocystin 4
• NCBI Official Symbol: NPHP4
• NCBI Official Synonym Symbols: POC10; SLSN4
• NCBI Protein Information: nephrocystin-4
• UniProt Protein Name: Nephrocystin-4
• Protein Family: Nephrocystin
• UniProt Gene Name: NPHP4
• UniProt Synonym Gene Names: KIAA0673

NCBI Description

This gene encodes a protein involved in renal tubular development and function. This protein interacts with nephrocystin, and belongs to a multifunctional complex that is localized to actin- and microtubule-based structures. Mutations in this gene are associated with nephronophthisis type 4, a renal disease, and with Senior-Loken syndrome type 4, a combination of nephronophthisis and retinitis pigmentosa. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

Involved in the organization of apical junctions; the function is proposed to implicate a NPHP1-4-8 module (PubMed:19755384, PubMed:21565611). Does not seem to be strictly required for ciliogenesis (PubMed:21565611). Required for building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells. Seems to recruit INT to basal bodies of motile cilia which subsequently interacts with actin-modifying proteins such as DAAM1 (). In cooperation with INVS may downregulate the canonical Wnt pathway and promote the Wnt-PCP pathway by regulating expression and subcellular location of disheveled proteins. Stabilizes protein levels of JADE1 and promotes its translocation to the nucleus leading to cooperative inhibition of canonical Wnt signaling (PubMed:21498478, PubMed:22654112). Acts as negative regulator of the hippo pathway by association with LATS1 and modifying LATS1-dependent phosphorylation and localization of WWTR1/TAZ (PubMed:21555462).