Rabbit anti-Human, Mouse NPFFR2 Polyclonal Antibody | anti-NPFFR2 antibody

NPFFR2 (Neuropeptide FF Receptor 2, G-protein Coupled Receptor 74, G-protein Coupled Receptor HLWAR77, Neuropeptide G-protein Coupled Receptor, GPR74, NPFF2, NPGPR)

Cat. Num. AAA646802

• Gene Names: NPFFR2; GPR74; NPFF2; NPGPR; HLWAR77
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: NPFFR2; Polyclonal Antibody; NPFFR2 (Neuropeptide FF Receptor 2; G-protein Coupled Receptor 74; G-protein Coupled Receptor HLWAR77; Neuropeptide G-protein Coupled Receptor; GPR74; NPFF2; NPGPR); Anti -NPFFR2 (Neuropeptide FF Receptor 2; anti-NPFFR2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NPFF2. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-NPFFR2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:500-1:1000
• Immunogen: Synthetic peptide corresponding to aa405-420 (ELVMEELKETTNSSEI) of human NPFF2, GenBank no. ref|NP_444264.1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NPFF2 antibody at 1:500 dilution. 20ug human brain lysate per well. Lane 1: Antibody detects 3 isoforms at 60kD and a 49kD doublet. Lane 2: Negative control (antibody preincubated with peptide).)

Related Product Information for anti-NPFFR2 antibody

NPFF2, a 60kD transmembrane protein, is a G-protein coupled NPFF receptor & shares homology with the seven trans-membrane domain receptor super family. It has a tissue-restricted distribution and consists of a 147-residue-N-terminal domain, 7 transmembrane domains and a C-terminal domain. It binds NPFF and might play a role in pain modulation and opiate tolerance. Reports suggest NPFF2 binds Prolactin releasing peptide and mediates its signaling. Northern Blot analysis detected a high expression of NPFF2 transcript in placenta, testis, thymus and small intestine with a moderate expression in other tissues.

Product Categories/Family for anti-NPFFR2 antibody

Antibodies; Abs to G Protein Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 221316654
• NCBI GeneID: 10886
• NCBI Accession #: NP_004876.2
• NCBI GenBank Nucleotide #: NM_004885.2
• UniProt Accession #: Q9Y5X5; Q96RV1; Q9NR49
• Molecular Weight: 60,270 Da
• NCBI Official Full Name: neuropeptide FF receptor 2 isoform 1
• NCBI Official Synonym Full Names: neuropeptide FF receptor 2
• NCBI Official Symbol: NPFFR2
• NCBI Official Synonym Symbols: GPR74; NPFF2; NPGPR; HLWAR77
• NCBI Protein Information: neuropeptide FF receptor 2; neuropeptide FF 2; G protein-coupled receptor 74; G-protein coupled receptor 74; G-protein coupled receptor HLWAR77; neuropeptide G-protein coupled receptor
• UniProt Protein Name: Neuropeptide FF receptor 2
• Protein Family: Neuropeptide FF receptor
• UniProt Gene Name: NPFFR2
• UniProt Synonym Gene Names: GPR74; NPFF2; NPGPR
• UniProt Entry Name: NPFF2_HUMAN

NCBI Description

This gene encodes a member of a subfamily of G-protein-coupled neuropeptide receptors. This protein is activated by the neuropeptides A-18-amide (NPAF) and F-8-amide (NPFF) and may function in pain modulation and regulation of the opioid system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]

Uniprot Description

Function: Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Subcellular location: Cell membrane; Multi-pass membrane protein.

Tissue specificity: Isoform 1 is abundant in placenta. Relatively highly expressed in thymus, testis, and small intestine. Expressed at low levels in several tissues including spleen, prostate, brain, heart, ovary, colon, kidney, lung, liver and pancreas and not expressed in skeletal muscle and leukocytes. Isoform 2 expression is highest in placenta (but at relatively low level compared to isoform 1). Very low level of expression in numerous tissues including adipose tissue and many brain regions. Isoform 3 is expressed in brain and heart and, at lower levels, in kidney, liver, lung and pancreas. Ref.1

Sequence similarities: Belongs to the G-protein coupled receptor 1 family.

Sequence caution: The sequence AAK58513.1 differs from that shown. Reason: Frameshift at position 503.