Goat anti-Human NPFFR1 Polyclonal Antibody | anti-NPFFR1 antibody

NPFFR1 (Neuropeptide FF receptor 1, FLJ10751, GPR147, NPFF1, NPFF1R1, OT7T022, G protein-coupled receptor 147, neuropeptide FF 1)

Cat. Num. AAA619104

• Gene Names: NPFFR1; NPFF1; GPR147; NPFF1R1; OT7T022; FLJ10751
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: NPFFR1; Polyclonal Antibody; NPFFR1 (Neuropeptide FF receptor 1; FLJ10751; GPR147; NPFF1; NPFF1R1; OT7T022; G protein-coupled receptor 147; neuropeptide FF 1); Anti -NPFFR1 (Neuropeptide FF receptor 1; anti-NPFFR1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human NPFFR1.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-NPFFR1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA, Western Blot.; Dilution: Peptide ELISA: 1:64,000.; Western Blot: 0.03-0.1ug/ml. Approx 48kD band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 47.8kD according to NP_071429.1). Optimal dilutions to be determined by the researcher.
• Immunogen: A synthetic peptide from the C-terminus of aa-RPSGSHKEAYSERP
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Chemiluminescence

(MBS619104 (0.03ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Product Categories/Family for anti-NPFFR1 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 11545887
• NCBI GeneID: 64106
• NCBI Accession #: NP_071429.1
• NCBI GenBank Nucleotide #: NM_022146.4
• UniProt Accession #: Q9GZQ6; Q8NGR0; Q96RN3; A2RRF0; Q5T6D8
• Molecular Weight: 47,819 Da
• NCBI Official Full Name: neuropeptide FF receptor 1
• NCBI Official Synonym Full Names: neuropeptide FF receptor 1
• NCBI Official Symbol: NPFFR1
• NCBI Official Synonym Symbols: NPFF1; GPR147; NPFF1R1; OT7T022; FLJ10751
• NCBI Protein Information: neuropeptide FF receptor 1; neuropeptide FF 1; OTTHUMP00000019749; G protein-coupled receptor 147; G-protein coupled receptor 147; RFamide-related peptide receptor OT7T022
• UniProt Protein Name: Neuropeptide FF receptor 1
• Protein Family: Neuropeptide FF receptor
• UniProt Gene Name: NPFFR1
• UniProt Synonym Gene Names: GPR147; NPFF1
• UniProt Entry Name: NPFF1_HUMAN

Uniprot Description

NPFFR1: Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 10q21-q22

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; neuropeptide receptor activity; peptide binding

Biological Process: synaptic transmission; neuropeptide signaling pathway; cellular response to hormone stimulus