Western Blot (WB)
((0. 03ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat NPFFR1 Polyclonal Antibody | anti-NPFFR1 antibody
Goat anti-NPFFR1 Antibody
Gene Names
NPFFR1; NPFF1; GPR147; NPFF1R1; OT7T022
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
NPFFR1; Polyclonal Antibody; Goat anti-NPFFR1 Antibody; neuropeptide FF receptor 1; FLJ10751; GPR147; NPFF1; NPFF1R1; OT7T022; G protein-coupled receptor 147; neuropeptide FF 1; NPFFR1 antibody; neuropeptide FF receptor 1 antibody; FLJ10751 antibody; GPR147 antibody; NPFF1 antibody; NPFF1R1 antibody; OT7T022 antibody; G protein-coupled receptor 147 antibody; neuropeptide FF 1 antibody; anti-NPFFR1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RPSGSHKEAYSERP
Sequence Length
430
Applicable Applications for anti-NPFFR1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 48kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 47.8kDa according to NP_071429.1). Recommended concentration: 0. 03-0.1ug/ml.
Western Blot: Approx 48kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 47.8kDa according to NP_071429.1). Recommended concentration: 0. 03-0.1ug/ml.
Immunogen
Peptide with sequence C-RPSGSHKEAYSERP, from the internal region of the protein sequence according to NP_071429.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0. 03ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 03ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,819 Da
NCBI Official Full Name
neuropeptide FF receptor 1
NCBI Official Synonym Full Names
neuropeptide FF receptor 1
NCBI Official Symbol
NPFFR1
NCBI Official Synonym Symbols
NPFF1; GPR147; NPFF1R1; OT7T022
NCBI Protein Information
neuropeptide FF receptor 1
UniProt Protein Name
Neuropeptide FF receptor 1
Protein Family
UniProt Gene Name
NPFFR1
UniProt Synonym Gene Names
GPR147; NPFF1
UniProt Entry Name
NPFF1_HUMAN
Uniprot Description
NPFFR1: Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. Belongs to the G-protein coupled receptor 1 family.
Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 10q21-q22
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity; neuropeptide receptor activity; peptide binding
Biological Process: cellular response to hormone stimulus; neuropeptide signaling pathway; synaptic transmission
Research Articles on NPFFR1
Similar Products
Product Notes
The NPFFR1 npffr1 (Catalog #AAA421604) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-NPFFR1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's NPFFR1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 48kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 47.8kDa according to NP_071429.1). Recommended concentration: 0. 03-0.1ug/ml. Researchers should empirically determine the suitability of the NPFFR1 npffr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RPSGSHKEAY SERP. It is sometimes possible for the material contained within the vial of "NPFFR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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