Western Blot (WB)
((0.3ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat NPAS4 Polyclonal Antibody | anti-NPAS4 antibody
Goat anti-NPAS4 Antibody
Gene Names
NPAS4; NXF; Le-PAS; PASD10; bHLHe79
Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
NPAS4; Polyclonal Antibody; Goat anti-NPAS4 Antibody; neuronal PAS domain protein 4; Le-PAS; NXF; PASD10; bHLHe79; HLH-PAS transcription factor NXF; PAS domain-containing protein 10; class E basic helix-loop-helix protein 79; neuronal PAS domain-containing protein 4; neuronal PAS4; NPAS4 antibody; neuronal PAS domain protein 4 antibody; Le-PAS antibody; NXF antibody; PASD10 antibody; bHLHe79 antibody; HLH-PAS transcription factor NXF antibody; PAS domain-containing protein 10 antibody; class E basic helix-loop-helix protein 79 antibody; neuronal PAS4 antibody; anti-NPAS4 antibody
Host
Goat
Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
CRFNTSKSLRRQS
Sequence Length
802
Applicable Applications for anti-NPAS4 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Approx 90kDa band observed in Human Brain (Frontal Cortex) lysates and in Mouse and Rat Brain lysates (calculated MW of 87.1kDa according to NP_849195.2). Recommended concentration: 0.3-1ug/ml.
Western Blot: Approx 90kDa band observed in Human Brain (Frontal Cortex) lysates and in Mouse and Rat Brain lysates (calculated MW of 87.1kDa according to NP_849195.2). Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence CRFNTSKSLRRQS, from the internal region of the protein sequence according to NP_849195.2.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.3ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Mouse (A) and Rat (B) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Mouse (A) and Rat (B) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Related Product Information for anti-NPAS4 antibody
266743
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
neuronal PAS domain-containing protein 4 isoform 1
NCBI Official Synonym Full Names
neuronal PAS domain protein 4
NCBI Official Symbol
NPAS4
NCBI Official Synonym Symbols
NXF; Le-PAS; PASD10; bHLHe79
NCBI Protein Information
neuronal PAS domain-containing protein 4
UniProt Protein Name
Neuronal PAS domain-containing protein 4
Protein Family
UniProt Gene Name
NPAS4
UniProt Synonym Gene Names
BHLHE79; NXF; PASD10; Neuronal PAS4; bHLHe79
UniProt Entry Name
NPAS4_HUMAN
NCBI Description
NXF is a member of the basic helix-loop-helix-PER (MIM 602260)-ARNT (MIM 126110)-SIM (see SIM2; MIM 600892) (bHLH-PAS) class of transcriptional regulators, which are involved in a wide range of physiologic and developmental events (Ooe et al., 2004 [PubMed 14701734]).[supplied by OMIM, Mar 2008]
Uniprot Description
NPAS4: Acts as a transcriptional activator in the presence of ARNT. Can activate the CME (CNS midline enhancer) element and the expression of the drebrin gene.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: nucleus; transcription factor complex
Molecular Function: protein heterodimerization activity
Biological Process: positive regulation of transcription from RNA polymerase II promoter; regulation of inhibitory postsynaptic membrane potential; transcription from RNA polymerase II promoter
Research Articles on NPAS4
Similar Products
Product Notes
The NPAS4 npas4 (Catalog #AAA422570) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-NPAS4 Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's NPAS4 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 90kDa band observed in Human Brain (Frontal Cortex) lysates and in Mouse and Rat Brain lysates (calculated MW of 87.1kDa according to NP_849195.2). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the NPAS4 npas4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CRFNTSKSLR RQS. It is sometimes possible for the material contained within the vial of "NPAS4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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