Western Blot (WB)
((1ug/ml) staining of MASM lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. CDK4 served as a loading control. Data provided Dr. B. Lasseque, Emory University, Atlanta, USA)
Goat NOX1 Polyclonal Antibody | anti-NOX1 antibody
Goat anti-NOX1 Antibody
Western Blot: Approx 65kDa band observed in Human and Mouse Colon lysates (calculated MW of 64.9kDa according to NP_008983.2). The 65kDa was also seen in cultured transgenic Mouse Aortic Smooth Muscle cells (MASM), but not to be seen at endogenous levels in MASM. Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of MASM lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. CDK4 served as a loading control. Data provided Dr. B. Lasseque, Emory University, Atlanta, USA)
Western Blot (WB)
((1ug/ml) staining of MASM lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. CDK4 served as a loading control. Data provided Dr. B. Lasseque, Emory University, Atlanta, USA)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the NADPH oxidase family of enzymes responsible for the catalytic one-electron transfer of oxygen to generate superoxide or hydrogen peroxide. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]
Uniprot Description
NOX1: NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.-.-.-; Membrane protein, multi-pass; Membrane protein, integral; Motility/polarity/chemotaxis; Oxidoreductase; Channel, misc.
Chromosomal Location of Human Ortholog: Xq22
Cellular Component: cell junction; early endosome; integral to membrane; NADPH oxidase complex; plasma membrane
Molecular Function: metal ion binding; NADP binding; protein binding; Rac GTPase binding; superoxide-generating NADPH oxidase activity; voltage-gated proton channel activity
Biological Process: angiogenesis; cell migration; extracellular matrix organization and biogenesis; hydrogen peroxide metabolic process; inflammatory response; intracellular pH elevation; NADP metabolic process; positive regulation of cell proliferation; positive regulation of integrin biosynthetic process; positive regulation of JNK cascade; positive regulation of smooth muscle cell proliferation; proton transport; regulation of blood pressure; regulation of systemic arterial blood pressure by renin-angiotensin; respiratory burst; signal transduction; superoxide metabolic process; superoxide release
Research Articles on NOX1
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Product Notes
The NOX1 nox1 (Catalog #AAA422878) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-NOX1 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's NOX1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 65kDa band observed in Human and Mouse Colon lysates (calculated MW of 64.9kDa according to NP_008983.2). The 65kDa was also seen in cultured transgenic Mouse Aortic Smooth Muscle cells (MASM), but not to be seen at endogenous levels in MASM. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the NOX1 nox1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DKATDIVTGL KQK. It is sometimes possible for the material contained within the vial of "NOX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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