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Western Blot (WB) (Western BlotPositive WB detected in: Jurkat whole cell lysateAll lanes: NOVA1 antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53, 20, 50, 51 KDaObserved band size: 53, 50 KDa)

Rabbit NOVA1 Polyclonal Antibody | anti-NOVA1 antibody

NOVA1 Antibody

Gene Names
NOVA1; Nova-1
Reactivity
Human, Rat, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
>95%, Protein G purified
Synonyms
NOVA1; Polyclonal Antibody; NOVA1 Antibody; RNA-binding protein Nova-1; Neuro-oncological ventral antigen 1; Onconeural ventral antigen 1; Paraneoplastic Ri antigen; Ventral neuron-specific protein 1; anti-NOVA1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
507
Applicable Applications for anti-NOVA1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:5000
IHC: 1:20-1:200
Immunogen
Recombinant Human RNA-binding protein Nova-1 protein (6-174AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Jurkat whole cell lysateAll lanes: NOVA1 antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53, 20, 50, 51 KDaObserved band size: 53, 50 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: Jurkat whole cell lysateAll lanes: NOVA1 antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53, 20, 50, 51 KDaObserved band size: 53, 50 KDa)

Immunohistochemistry (IHC)

(IHC image of MBS7105805 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC) (IHC image of MBS7105805 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Related Product Information for anti-NOVA1 antibody
May regulate RNA splicing or metabolism in a specific subset of developing neurons.
Product Categories/Family for anti-NOVA1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,253 Da
NCBI Official Full Name
RNA-binding protein Nova-1 isoform 1
NCBI Official Synonym Full Names
NOVA alternative splicing regulator 1
NCBI Official Symbol
NOVA1
NCBI Official Synonym Symbols
Nova-1
NCBI Protein Information
RNA-binding protein Nova-1
UniProt Protein Name
RNA-binding protein Nova-1
Protein Family
UniProt Gene Name
NOVA1

NCBI Description

This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

May regulate RNA splicing or metabolism in a specific subset of developing neurons.

Research Articles on NOVA1

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Product Notes

The NOVA1 nova1 (Catalog #AAA7105805) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NOVA1 Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NOVA1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:5000 IHC: 1:20-1:200. Researchers should empirically determine the suitability of the NOVA1 nova1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOVA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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