Western Blot (WB)
(Anti- NOTCH4 antibody, MBS176101, Western blottingAll lanes: Anti NOTCH4 (MBS176101) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: SMMC Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugPredicted bind size: 210KDObserved bind size: 210KD )
Rabbit anti-Human NOTCH4 Polyclonal Antibody | anti-NOTCH4 antibody
Anti-NOTCH4 antibody
Western Blot (WB)
(Anti- NOTCH4 antibody, MBS176101, Western blottingAll lanes: Anti NOTCH4 (MBS176101) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: SMMC Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugPredicted bind size: 210KDObserved bind size: 210KD )
Western Blot (WB)
(Anti- NOTCH4 antibody, MBS176101, Western blottingAll lanes: Anti NOTCH4 (MBS176101) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: SMMC Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugPredicted bind size: 210KDObserved bind size: 210KD )
Background: NOTCH4(NOTCH, DROSOPHILA, HOMOLOG OF, 4) also known as INT3 or NOTCH3, FORMERLY, is a member of the Notch family. In situ hybridization revealed that Notch4 transcripts are primarily restricted to endothelial cells in embryonic and adult life, suggesting a specific role forNotch4 during development of vertebrate endothelium. The sequences of the mouse and human NOTCH4 proteins are 82% identical. Northern blot analysis revealed that NOTCH4(S) is the major transcript and is expressed in a wide variety of tissues. Fluorescence in situ hybridization confirmed the location of the NOTCH4 gene at chromosome 6p21.3. In linkage disequilibrium mapping of the MHC region in 80 British parent-offspring trios, Wei and Hemmings(2000) found that NOTCH4 was highly associated with schizophrenia. Repression of Notch4 resolved ataxia and reversed the disease progression, demonstrating that Notch4 is not only sufficient to induce but also required to sustain the disease.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. This gene may be associated with susceptibility to schizophrenia in a small portion of cases. An alternative splice variant has been described but its biological nature has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
NOTCH4: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system. Heterodimer of a C-terminal fragment N(TM) and a N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH4. Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes. Belongs to the NOTCH family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; cell surface; integral to plasma membrane; extracellular region; plasma membrane; cytosol; nucleus
Molecular Function: protein binding; protein heterodimerization activity; calcium ion binding; receptor activity
Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; positive regulation of transcription, DNA-dependent; Notch receptor processing; morphogenesis of a branching structure; cell fate determination; activation of Notch receptor target transcription factor; patterning of blood vessels; negative regulation of cell differentiation; embryonic development; negative regulation of endothelial cell differentiation; mammary gland development; hemopoiesis; gene expression; endothelial cell morphogenesis; cell differentiation
Research Articles on NOTCH4
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Product Notes
The NOTCH4 notch4 (Catalog #AAA176101) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NOTCH4 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NOTCH4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the NOTCH4 notch4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOTCH4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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