Western Blot (WB)
(Western Blot: Sample: Porcine Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Human NME3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human Non Metastatic Cells 3, Protein NM23A Expressed In (NME3) Polyclonal Antibody | anti-NME3 antibody
Polyclonal Antibody to Non Metastatic Cells 3, Protein NM23A Expressed In (NME3)
Gene Names
NME3; NDPKC; NDPK-C; NM23H3; DR-nm23; NM23-H3; c371H6.2
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Non Metastatic Cells 3; Protein NM23A Expressed In (NME3); Polyclonal Antibody; Polyclonal Antibody to Non Metastatic Cells 3; anti-NME3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against NME3. It has been selected for its ability to recognize NME3 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MICLVLTIFA NLFPAACTGA HERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYA ELRERPFYGR LVKYMASGPV VAMVWQGLDV VRTSRALIGA TNPADAPPGT IRGDFCIEVG KNLIHGSDSV
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MICLVLTIFA NLFPAACTGA HERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYA ELRERPFYGR LVKYMASGPV VAMVWQGLDV VRTSRALIGA TNPADAPPGT IRGDFCIEVG KNLIHGSDSV
Applicable Applications for anti-NME3 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant NME3 (Met1~Tyr168) expressed in E.coli.
Cross Reactivity
Human, Mouse, Pig
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2082146
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Porcine Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Human NME3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Porcine Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Human NME3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,015 Da
NCBI Official Full Name
nucleoside diphosphate kinase 3
NCBI Official Synonym Full Names
NME/NM23 nucleoside diphosphate kinase 3
NCBI Official Symbol
NME3
NCBI Official Synonym Symbols
NDPKC; NDPK-C; NM23H3; DR-nm23; NM23-H3; c371H6.2
NCBI Protein Information
nucleoside diphosphate kinase 3
UniProt Protein Name
Nucleoside diphosphate kinase 3
Protein Family
UniProt Gene Name
NME3
UniProt Synonym Gene Names
NDK 3; NDP kinase 3; NDPKC
Uniprot Description
NME3: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Probably has a role in normal hematopoiesis by inhibition of granulocyte differentiation and induction of apoptosis. Belongs to the NDK family.
Protein type: Apoptosis; EC 2.7.4.6; Kinase, nucleoside diphosphate; Kinase, other; Motility/polarity/chemotaxis; NDK family; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Other group
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: cytosol; intracellular; mitochondrion
Molecular Function: ATP binding; metal ion binding; nucleoside diphosphate kinase activity; protein binding
Biological Process: apoptosis; CTP biosynthetic process; GTP biosynthetic process; nucleobase, nucleoside and nucleotide interconversion; nucleoside diphosphate phosphorylation; regulation of apoptosis; UTP biosynthetic process
Research Articles on NME3
Similar Products
Product Notes
The NME3 nme3 (Catalog #AAA2017013) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Non Metastatic Cells 3, Protein NM23A Expressed In (NME3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Non Metastatic Cells 3, Protein NM23A Expressed In (NME3) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NME3 nme3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MICLVLTIFA NLFPAACTGA HERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYA ELRERPFYGR LVKYMASGPV VAMVWQGLDV VRTSRALIGA TNPADAPPGT IRGDFCIEVG KNLIHGSDSV. It is sometimes possible for the material contained within the vial of "Non Metastatic Cells 3, Protein NM23A Expressed In (NME3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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