Rabbit anti-Human NOLC1 Polyclonal Antibody | anti-NOLC1 antibody

Anti-NOLC1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8110249

• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: NOLC1; Polyclonal Antibody; Anti-NOLC1 Antibody; Rabbit Polyclonal; NOLC1 Antibody; Rabbit PAb; Antigen Affinity Purified; nucleolar and coiled-body phosphoprotein 1; Anti-NOPP130 Antibody; Anti-NOPP140 Antibody; Anti-NS5ATP13 Antibody; Anti-P130 Antibody; anti-NOLC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NOLC1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-NOLC1 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:50-1:200; ICC/IF: 1:100-1:500
• Immunogen: E Coli-derived Human NOLC1 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human NOLC1 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human NOLC1 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human NOLC1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of NOLC1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NOLC1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.)

Related Product Information for anti-NOLC1 antibody

Nucleolar and coiled-body phosphoprotein 1 (NOLC1) is a phosphoprotein that transiently associates with the mature nucleolar H/ACA and C/D box small nucleolar ribonucleoproteins (snoRNPs). NOLC1 plays an essential role in the synthesis of rRNA and the biosynthesis of ribosomes. NOLC1 is responsible for the nucleolus construction and rRNA synthesis interacted with TRF2 and mediated the shuttle of TRF2 between the nucleolus and nucleus. Studies suggest that NOLC1 is crucial for normal cell growth, and plays a role in the regulation of tumorigenesis of nasopharyngeal carcinoma (NPC) and demonstrate that both NOLC1 and tumor protein 53 work synergistically to activate the MDM2 promoter in NPC cells. DNA methylation is a key mechanism of silenced NOLC1 expression in human hepatocellular carcinoma cells, and NOLC1 gene hypermethylation of the four CpG dinucleotides is a potential biomarker for hepatocellular carcinoma.

References

Yuan F, et al. (2018) Nucleolar trf2 attenuated nucleolus stress-induced hcc cell-cycle arrest by altering rrna synthesis. Cell Death Dis 9 (5): 518.Gao X, et al. (2011) Identification of nucleolar and coiled-body phosphoprotein 1 (nolc1) minimal promoter regulated by nf-kappab and creb. BMB Rep 44 (1): 70-75.Duan X, et al. (2013) Methylation of nucleolar and coiled-body phosphoprotein 1 is associated with the mechanism of tumorigenesis in hepatocellular carcinoma. Oncol Rep 30 (5): 2220-2228.