Rabbit NOD5 Polyclonal Antibody | anti-NLRX1 antibody

NOD5 Antibody

Cat. Num. AAA151491

• Gene Names: NLRX1; NOD5; NOD9; NOD26; DLNB26; CLR11.3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: NOD5 Antibody is affinity chromatography purified via peptide column.
• Synonyms: NOD5; Polyclonal Antibody; NOD5 Antibody; NOD9; NOD26; DLNB26; CLR11.3; NLR family member X1; Nucleotide-binding oligomerization domain protein 26; anti-NLRX1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: NOD5 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 975

• Applicable Applications for anti-NLRX1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: NOD5 antibody can be used for detection of NOD5 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: NOD5 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human NOD5.
• Buffer: NOD5 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: NOD5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of NOD5 in rat spleen tissue cell lysate with NOD5 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of NOD5 in rat spleen tissue with NOD5 antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of NOD5 in rat spleen tissue with NOD5 antibody at 20 μg/mL.)

Related Product Information for anti-NLRX1 antibody

NOD5 Antibody: NOD5, also known as NLRX1, is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD5 localizes to the mitochondrial outer membrane and interacts with the virus-induced signaling adapter protein VISA. Unlike a subset of NOD-like receptors (NLRs) such as NOD1 and NOD2 which trigger pro-inflammatory cascades, and other NLRs that induce the caspase 1 inflammasome in response to immune challenges, NOD5 amplifies NF-κB and JNK pathways by inducing reactive oxygen species production.

NCBI and Uniprot Product Information

• NCBI GI #: 25777608
• NCBI GeneID: 79671
• NCBI Accession #: NP_078894
• NCBI GenBank Nucleotide #: NM_024618.3
• UniProt Accession #: Q86UT6; Q7RTR3; Q96D51; Q9H724; A8K6Q1; B3KPK2; B3KTA2
• Molecular Weight: 101,876 Da
• NCBI Official Full Name: NLR family member X1
• NCBI Official Synonym Full Names: NLR family member X1
• NCBI Official Symbol: NLRX1
• NCBI Official Synonym Symbols: NOD5; NOD9; NOD26; DLNB26; CLR11.3
• NCBI Protein Information: NLR family member X1; NLR family, X1; NOD-like receptor X1; caterpiller protein 11.3; nucleotide-binding oligomerization domain protein 5; nucleotide-binding oligomerization domain protein 9; nucleotide-binding oligomerization domain protein 26; nucleotide-binding oligomerization domain, leucine rich repeat containing X1
• UniProt Protein Name: NLR family member X1
• Protein Family: NLR family
• UniProt Gene Name: NLRX1
• UniProt Synonym Gene Names: NOD26; NOD5; NOD9; CLR11.3
• UniProt Entry Name: NLRX1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the NLR family and localizes to the outer mitochondrial membrane. The encoded protein is a regulator of mitochondrial antivirus responses. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2013]

Uniprot Description

NLRX1: Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction (PubMed:18200010). Has no inhibitory function on NF- Kappa-B and type 1 interferon signaling pathways, but enhances NF- Kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species (PubMed:18219313). Interacts with MAVS. Ubiquitously expressed. Strongest expression in mammary gland, heart and muscle. Detected in HeLa, HEK293T, THP-1, HL-60, Raji, and Jurkat cell lines. Belongs to the NLRP family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: mitochondrial outer membrane

Molecular Function: protein binding; ATP binding

Biological Process: viral reproduction; negative regulation of innate immune response; negative regulation of inflammatory response; negative regulation of interleukin-6 production; innate immune response; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interferon type I production; negative regulation of interferon-beta production