Western Blot (WB)
(Western blot analysis of extracts from Myeloma cells, using Nociceptin Antibody. The lane on the left was treated with blocking peptide.Observed bands: 25 kDa.)
Rabbit Nociceptin Polyclonal Antibody | anti-PNOC antibody
Nociceptin Antibody
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
IF: 1:100-1:500
ICC: 1:100-1:500
ELISA(peptide): 1:20,000-1:40,000
Western Blot (WB)
(Western blot analysis of extracts from Myeloma cells, using Nociceptin Antibody. The lane on the left was treated with blocking peptide.Observed bands: 25 kDa.)
Western Blot (WB)
(Western blot analysis of extracts from Myeloma cells, using Nociceptin Antibody. The lane on the left was treated with blocking peptide.Observed bands: 25 kDa.)
Western Blot (WB)
(Western blot analysis of extracts from Rat brain, using Nociceptin Antibody. The lane on the left was treated with blocking peptide.Observed bands: 25 kDa.)
Western Blot (WB)
(Western blot analysis of extracts from Rat brain, using Nociceptin Antibody. The lane on the left was treated with blocking peptide.Observed bands: 25 kDa.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612144 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612144 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612144 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612144 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Post Translational Modifications: Specific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin.The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
Subcellular Location: Secreted.
Tissue Specificity: Predominantly expressed in the brain and spinal cord. Also expressed and secreted by peripheral blood neutrophils following degranulation.
Similarity: Belongs to the opioid neuropeptide precursor family.
NCBI and Uniprot Product Information
Predicted Molecular Weight: (Calculated)20kDa.
NCBI Description
This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include nociceptin, nocistatin, and orphanin FQ2 (OFQ2). Nociceptin, also known as orphanin FQ, is a 17-amino acid neuropeptide that binds to the nociceptin receptor to induce increased pain sensitivity, and may additionally regulate body temperature, learning and memory, and hunger. Another product of the encoded preproprotein, nocistatin, may inhibit the effects of nociceptin. [provided by RefSeq, Jul 2015]
Uniprot Description
PNOC: Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development. Belongs to the opioid neuropeptide precursor family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 8p21
Cellular Component: extracellular region
Molecular Function: neuropeptide hormone activity; protein binding; opioid peptide activity
Biological Process: synaptic transmission; neuropeptide signaling pathway; sensory perception; signal transduction
Research Articles on PNOC
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Product Notes
The PNOC pnoc (Catalog #AAA9612144) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nociceptin Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's Nociceptin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 IF: 1:100-1:500 ICC: 1:100-1:500 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the PNOC pnoc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nociceptin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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