Rabbit anti-Human nNOS Polyclonal Antibody | anti-nNOS antibody

nNOS, Phosphorylated (S1417) (NOS1, Nitric oxide synthase, brain, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1, bNOS) (PE)

Cat. Num. AAA6325916

• Gene Names: NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• Reactivity: Human
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: nNOS; Polyclonal Antibody; Phosphorylated (S1417) (NOS1; Nitric oxide synthase; brain; Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; Peptidyl-cysteine S-nitrosylase NOS1; bNOS) (PE); NOS1; bNOS; anti-nNOS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-nNOS antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Phospho-nNOS-S1417 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 1403~1432 amino acids surrounding S1417 of human nNOS.
• Conjugate: PE
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-Phospho-nNOS-S1417 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Related Product Information for anti-nNOS antibody

Three isoforms of nitric oxide synthase (NOS) have been identified. All are homodimers with subunits of 130-160 kDa. All have binding sites for NADPH, FAD, and FMN near the carboxyl terminus (the reductase domain), and binding sites for tetrahydrobiopterin (BH4) and heme near the amino terminus (the oxygenase domain). The reductase and oxygenase domains are linked by a calmodulin (CaM) binding site. Occupation of this site facilitates electron transfer from the cofactors in the reductase domain to heme during nitric oxide production. NOS catalyzes the conversion of arginine to citrulline and nitric oxide (NO). Neuronal nitric oxide synthase (nNOS, bNOS, cNOS, Type I) is associated with the post-synaptic density protein (PSD-95) in the neuronal membrane. In response to increased intracellular Ca2+, nNOS interacts with CaM. The Ca2+ CaM complex, in combination with BH4, binds to nNOS and induces its translocation from the plasma membrane to the cytoplasm. The dephosphorylation of nNOS by calcineurin initiates the production NO. NO activates guanylyl cyclase (GC) and activates the various cGMP regulated signaling pathways. nNOS is in activated by phosphorylation by protein kinase A (PKA) or protein kinase C (PKC).

Product Categories/Family for anti-nNOS antibody

Antibodies; Nitric Oxide

References

Laas, K., et.al., Psychopharmacology (Berl.) 209 (3), 255-261 (2010). Darrah, R., et.al., Physiol. Genomics 41 (1), 71-77 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 10835173
• NCBI GeneID: 4842
• NCBI Accession #: NP_000611.1
• NCBI GenBank Nucleotide #: NM_000620.4
• UniProt Accession #: P29475; O75713; E9PH30
• Molecular Weight: 160,970 Da
• NCBI Official Full Name: nitric oxide synthase, brain isoform 1
• NCBI Official Synonym Full Names: nitric oxide synthase 1 (neuronal)
• NCBI Official Symbol: NOS1
• NCBI Official Synonym Symbols: NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• NCBI Protein Information: nitric oxide synthase, brain; NOS type I; neuronal NOS; constitutive NOS; peptidyl-cysteine S-nitrosylase NOS1
• UniProt Protein Name: Nitric oxide synthase, brain
• UniProt Gene Name: NOS1
• UniProt Synonym Gene Names: N-NOS; nNOS
• UniProt Entry Name: NOS1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described.

Protein type: Amino Acid Metabolism - arginine and proline; EC 1.14.13.39; Oxidoreductase

Chromosomal Location of Human Ortholog: 12q24.22

Cellular Component: photoreceptor inner segment; protein complex; cytoskeleton; sarcoplasmic reticulum; mitochondrion; perinuclear region of cytoplasm; cytoplasm; dendritic spine; synapse; cytosol; sarcolemma; lipid raft

Molecular Function: calmodulin binding; protein binding; sodium channel regulator activity; FAD binding; NADPH-hemoprotein reductase activity; FMN binding; nitric-oxide synthase activity; iron ion binding; heme binding; oxidoreductase activity; NADP binding; cadmium ion binding

Biological Process: interaction with host; negative regulation of serotonin uptake; negative regulation of calcium ion transport; striated muscle contraction; positive regulation of transcription, DNA-dependent; negative regulation of potassium ion transport; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; positive regulation of vasodilation; response to heat; negative regulation of hydrolase activity; exogenous drug catabolic process; response to hypoxia; peptidyl-cysteine S-nitrosylation; arginine catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; negative regulation of blood pressure; nitric oxide biosynthetic process; blood coagulation; nitric oxide mediated signal transduction; multicellular organismal response to stress; myoblast fusion; neurotransmitter biosynthetic process

Disease: Pyloric Stenosis, Infantile Hypertrophic, 1