Sheep nNOS, aa1409-1429 Polyclonal Antibody | anti-NOS1 antibody

nNOS, aa1409-1429 (Neuronal Nitric Oxide Synthase, bNOS)

Cat. Num. AAA6011120

• Gene Names: NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• Reactivity: Guinea Pig, Rabbit, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Serum; Serum
• Synonyms: nNOS; aa1409-1429; Polyclonal Antibody; aa1409-1429 (Neuronal Nitric Oxide Synthase; bNOS); Anti -nNOS; anti-NOS1 antibody

• Host: Sheep
• Reactivity: Guinea Pig, Rabbit, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat bNOS (neuronal). Not yet tested for crossreactivity to iNOS or eNOS. Species Crossreactivity: guinea-pig and rabbit.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a lyophilized powder. Contains no preservatives. Reconstitute with 100ul sterile ddH2O.

• Applicable Applications for anti-NOS1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Immunohistochemistry: 1:500-1:1000
• Immunogen: Synthetic peptide corresponding to aa1409-1429, H-RSESIAFIEESKKDADEVFSS-NH2, of the neuronal form of NOS from rat cerebellum, conjugated to KLH.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Staining of rabbit brain stem neurons and fibers.)

Testing Data

(Staining of guinea pig ileum.)

Testing Data

(Staining of guinea pig coeliac ganglia.)

Product Categories/Family for anti-NOS1 antibody

Antibodies; Abs to Nitric Oxide

NCBI and Uniprot Product Information

• NCBI GI #: 10835173
• NCBI GeneID: 4842
• NCBI Accession #: NP_000611.1
• NCBI GenBank Nucleotide #: NM_000620.4
• UniProt Accession #: P29475; O75713; E9PH30
• Molecular Weight: 160,970 Da
• NCBI Official Full Name: nitric oxide synthase, brain isoform 1
• NCBI Official Synonym Full Names: nitric oxide synthase 1 (neuronal)
• NCBI Official Symbol: NOS1
• NCBI Official Synonym Symbols: NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• NCBI Protein Information: nitric oxide synthase, brain; NOS type I; neuronal NOS; constitutive NOS; peptidyl-cysteine S-nitrosylase NOS1
• UniProt Protein Name: Nitric oxide synthase, brain
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NOS1
• UniProt Synonym Gene Names: N-NOS; nNOS
• UniProt Entry Name: NOS1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described.

Protein type: Oxidoreductase; EC 1.14.13.39; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 12q24.22

Cellular Component: cytoskeleton; photoreceptor inner segment; protein complex; sarcoplasmic reticulum; mitochondrion; perinuclear region of cytoplasm; cytoplasm; dendritic spine; synapse; cytosol; sarcolemma; lipid raft

Molecular Function: calmodulin binding; protein binding; FAD binding; sodium channel regulator activity; FMN binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; iron ion binding; heme binding; oxidoreductase activity; NADP binding; cadmium ion binding

Biological Process: negative regulation of serotonin uptake; interaction with host; negative regulation of calcium ion transport; striated muscle contraction; negative regulation of potassium ion transport; positive regulation of transcription, DNA-dependent; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; positive regulation of vasodilation; response to heat; negative regulation of hydrolase activity; exogenous drug catabolic process; peptidyl-cysteine S-nitrosylation; response to hypoxia; arginine catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; negative regulation of blood pressure; blood coagulation; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; multicellular organismal response to stress; myoblast fusion; neurotransmitter biosynthetic process

Disease: Pyloric Stenosis, Infantile Hypertrophic, 1