Rabbit NMDAR2A Polyclonal Antibody | anti-GRIN2A antibody

Anti-NMDAR2A antibody

Cat. Num. AAA176362

• Gene Names: GRIN2A; LKS; EPND; FESD; NR2A; GluN2A; NMDAR2A
• Reactivity: Mouse, Rat; Predicted to work with:Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: NMDAR2A; Polyclonal Antibody; Anti-NMDAR2A antibody; anti-GRIN2A antibody

• Host: Rabbit
• Reactivity: Mouse, Rat; Predicted to work with:Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 1464

• Applicable Applications for anti-GRIN2A antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western blot,; Concentration:0.1-0.5 ug/ml; Tested Species:Mouse; Predicted Species:Human; ; Immunohistochemistry(Paraffin-embedded Section); Concentration:0.5-1 ug/ml; Tested Species:Rat; Predicted Species: Human, Mouse; Antigen Retrieval:By Heat; ; Tested Species:In-house tested species with positive results; Predited Species:Species predicted to be fit for the product based on sequence similarities; By Heat: Boiling the paraffin sections in mM citrate buffer,pH6.0,for mins is required for the staining of formalin/paraffin sections; Other applications have not been tested.; Optimal dilutions should be determined end users.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human NMDAR2A (1360-1376aa, DHTSDNPFLHSHRDDQR), different from the related mouse sequence by three amino acids, and from the related rat sequences by four amino acids.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-NMDAR2A antibody, MBS176362, Western blottingAll lanes: Anti NMDAR2A (MBS176362) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Mouse Brain Tissue Lysate at 50ugPredicted bind size: 165KDObserved bind size: 165KD )

Immunohistochemistry (IHC)

(Anti-NMDAR2A antibody, MBS176362, IHC(P)IHC(P): Rat Brain Tissue )

Related Product Information for anti-GRIN2A antibody

GRIN2A is also known as N-methyl-D-aspartate receptor channel, subunit epsilon-1(NMDAR2A). This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 14285603
• NCBI GeneID: 2903
• UniProt Accession #: Q12879; O00669; Q17RZ6
• NCBI Official Full Name: Glutamate receptor ionotropic, NMDA 2A
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• NCBI Official Symbol: GRIN2A
• NCBI Official Synonym Symbols: LKS; EPND; FESD; NR2A; GluN2A; NMDAR2A
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 2A; N-methyl D-aspartate receptor subtype 2A; N-methyl-D-aspartate receptor subunit 2A; N-methyl-D-aspartate receptor channel, subunit epsilon-1
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 2A
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: GRIN2A
• UniProt Synonym Gene Names: NMDAR2A; GluN2A; NMDAR2A; NR2A; hNR2A
• UniProt Entry Name: NMDE1_HUMAN

NCBI Description

This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

NMDAR2A: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, ligand-gated

Chromosomal Location of Human Ortholog: 16p13.2

Cellular Component: presynaptic membrane; postsynaptic membrane; synaptic vesicle; cell surface; integral to plasma membrane; endoplasmic reticulum; dendrite; plasma membrane; cell junction; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; zinc ion binding; N-methyl-D-aspartate selective glutamate receptor activity; calcium channel activity

Biological Process: startle response; positive regulation of apoptosis; regulation of synaptic plasticity; sensory perception of pain; dopamine metabolic process; synaptic transmission; protein localization; learning and/or memory; transport; response to wounding; visual learning; serotonin metabolic process; response to drug; synaptic transmission, glutamatergic; glutamate signaling pathway; response to amphetamine; sleep; memory; response to ethanol; neurogenesis; ionotropic glutamate receptor signaling pathway; directional locomotion; regulation of sensory perception of pain; regulation of excitatory postsynaptic membrane potential; negative regulation of protein catabolic process

Disease: Epilepsy, Focal, With Speech Disorder And With Or Without Mental Retardation