Western Blot (WB)
(Anti-NMDAR1 antibody, MBS175244, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Rat Liver Tissue LysateLane 4: Rat Heart Tissue LysateLane 5: MM453 Cell LysateLane 6: MM231 Cell LysateLane 7: HELA Cell Lysate )
Rabbit NMDAR1 Polyclonal Antibody | anti-GRIN1 antibody
Anti-NMDAR1 antibody
Predicted to work with: Mouse
Predicted to work with: Mouse
Concentration: 0.1-0.5 ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Antigen Retrieval: n/a
Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1 ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Antigen Retrieval: By Heat
Test Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formal/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Western Blot (WB)
(Anti-NMDAR1 antibody, MBS175244, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Rat Liver Tissue LysateLane 4: Rat Heart Tissue LysateLane 5: MM453 Cell LysateLane 6: MM231 Cell LysateLane 7: HELA Cell Lysate )
Western Blot (WB)
(Anti-NMDAR1 antibody, MBS175244, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Rat Liver Tissue LysateLane 4: Rat Heart Tissue LysateLane 5: MM453 Cell LysateLane 6: MM231 Cell LysateLane 7: HELA Cell Lysate )
Immunohistochemistry (IHC)
(Anti-NMDAR1 antibody, MBS175244, IHC(P)IHC(P): Rat Brain Tissue )
Immunohistochemistry (IHC)
(Anti-NMDAR1 antibody, MBS175244, IHC(P)IHC(P): Rat Brain Tissue )
Background: The NMDA receptor(NMDAR) is a specific type of ionotropic glutamate receptor. NMDA(N-methyl D-aspartate) is the name of a selective agonist that binds to NMDA receptors but not to other glutamate receptors. Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. NMDAR1 gene is mapped to 9q34.3 and encodes a 938-amino acid protein which showed high evolutionary conservation in structure and physiologic properties. It consists of 21 exons distributed over about 31 kb. Three of the exons that are alternatively spliced in the rat and which produce 8 isoforms in that species were also present in the human sequence. The promoter region contained 2 DNA binding sites for the homeobox proteins 'even-skipped'. The gene is a candidate for the site of the mutation in torsion dystonia.The NMDA receptor is a non-specific cation channel and thus directly contributes to excitatory synaptic transmission by depolarizing the postsynaptic cell. NMDA receptors are modulated by a number of endogenous and exogenous compounds and play a key role in a wide range of physiologic and pathologic processes, such as excitotoxicity.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
NMDAR1: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. Three alternatively-spliced isoforms have been described.
Protein type: Channel, ligand-gated; Membrane protein, multi-pass; Membrane protein, integral; Channel, calcium
Chromosomal Location of Human Ortholog: 9q34.3
Cellular Component: neuron projection; cell surface; endoplasmic reticulum; integral to plasma membrane; postsynaptic density; dendrite; dendritic spine; terminal button; excitatory synapse; N-methyl-D-aspartate selective glutamate receptor complex; postsynaptic membrane; synaptic vesicle; plasma membrane; synapse; cell junction
Molecular Function: voltage-gated cation channel activity; neurotransmitter binding; glutamate receptor binding; calcium channel activity; calcium ion binding; calmodulin binding; protein binding; enzyme binding; glutamate binding; extracellular-glutamate-gated ion channel activity; protein heterodimerization activity; N-methyl-D-aspartate selective glutamate receptor activity; glycine binding
Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; male mating behavior; prepulse inhibition; adult locomotory behavior; positive regulation of apoptosis; regulation of dendrite morphogenesis; rhythmic process; response to morphine; sensory perception of pain; regulation of axonogenesis; calcium ion homeostasis; synaptic transmission; regulation of respiratory gaseous exchange; conditioned taste aversion; ephrin receptor signaling pathway; visual learning; negative regulation of neuron apoptosis; protein tetramerization; cation transport; synaptic transmission, glutamatergic; response to amphetamine; social behavior; respiratory gaseous exchange; pons maturation; cellular calcium ion homeostasis; regulation of membrane potential; response to ethanol; regulation of synaptogenesis; olfactory learning; long-term memory; suckling behavior; propylene metabolic process; ionotropic glutamate receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development; regulation of excitatory postsynaptic membrane potential; response to calcium ion
Disease: Mental Retardation, Autosomal Dominant 8
Research Articles on GRIN1
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Product Notes
The GRIN1 grin1 (Catalog #AAA175244) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NMDAR1 antibody reacts with Reacts with: Human, rat Predicted to work with: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NMDAR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot: Concentration: 0.1-0.5 ug/ml Tested Species: Hu, Rat Predicted Species: Ms Antigen Retrieval: Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1 ug/ml Tested Species: Hu, Rat Predicted Species: Ms Antigen Retrieval: By Heat Test Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formal/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the GRIN1 grin1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NMDAR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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