Rabbit anti-Human NLRP2 Polyclonal Antibody | anti-NLRP2 antibody

Rabbit anti-human NLRP2 polyclonal Antibody

Cat. Num. AAA1492110

• Gene Names: NLRP2; NBS1; PAN1; NALP2; PYPAF2; CLR19.9
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: NLRP2; Polyclonal Antibody; Rabbit anti-human NLRP2 polyclonal Antibody; Nucleotide-binding site protein 1; PYRIN domain and NACHT domain-containing protein 1; PYRIN-containing APAF1-like protein 2; NALP2; NBS1; PAN1; PYPAF2; anti-NLRP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 1062

• Applicable Applications for anti-NLRP2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:200-1000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human NACHT, LRR and PYD domains-containing protein 2 protein (1-220aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-50616 / sc-50617 / sc-50619
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: NLRP2 antibody at 12ug/ml+A431 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 121,119,97,118 kDaObserved band size: 121 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS1492110 at dilution 1:100)

Related Product Information for anti-NLRP2 antibody

Suppresses TNF- and CD40-induced NFKB1 activity at the level of the IKK complex, by inhibiting NFKBIA degradation induced by TNF. When associated with PYCARD, activates CASP1, leading to the secretion of mature proinflammatory cytokine IL1B. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.

NCBI and Uniprot Product Information

• NCBI GI #: 291463275
• NCBI GeneID: 55655
• NCBI Accession #: NP_001167552.1
• NCBI GenBank Nucleotide #: NM_001174081.1
• UniProt Accession #: Q9NX02; Q53FL5; Q59G09; Q8IXT0; Q9BVN5; Q9H6G6; Q9HAV9; Q9NWK3; B4DZL7; I3L0G4
• Molecular Weight: 117,883 Da
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 2 isoform 1
• NCBI Official Synonym Full Names: NLR family, pyrin domain containing 2
• NCBI Official Symbol: NLRP2
• NCBI Official Synonym Symbols: NBS1; PAN1; NALP2; PYPAF2; CLR19.9
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 2
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 2
• Protein Family: NACHT, LRR and PYD domains-containing protein
• UniProt Gene Name: NLRP2
• UniProt Synonym Gene Names: NALP2; NBS1; PAN1; PYPAF2
• UniProt Entry Name: NALP2_HUMAN

NCBI Description

NALP proteins, such as NALP2, are characterized by an N-terminal pyrin (MIM 608107) domain (PYD) and are involved in the activation of caspase-1 (CASP1; MIM 147678) by Toll-like receptors (see TLR4; MIM 603030). They may also be involved in protein complexes that activate proinflammatory caspases (Tschopp et al., 2003 [PubMed 12563287]).[supplied by OMIM, Mar 2008]

Uniprot Description

NLRP2: Suppresses TNF- and CD40-induced NFKB1 activity at the level of the IKK complex, by inhibiting NFKBIA degradation induced by TNF. When associated with PYCARD, activates CASP1, leading to the secretion of mature proinflammatory cytokine IL1B. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Belongs to the NLRP family. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: cytoplasm; Golgi apparatus; intracellular membrane-bound organelle; nuclear chromosome, telomeric region

Molecular Function: ATP binding; protein binding; Pyrin domain binding

Biological Process: inflammatory response; innate immune response; positive regulation of caspase activity; positive regulation of interleukin-1 beta secretion