Western Blot (WB)
(Western blot analysis of extracts from Mouse kidney tissue, using NIT2 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit NIT2 Polyclonal Antibody | anti-NIT2 antibody
NIT2 Antibody
Gene Names
NIT2; HEL-S-8a
Reactivity
Human, Mouse, RatPredicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
NIT2; Polyclonal Antibody; NIT2 Antibody; CUA002; MGC111199; NIT 2; Nit protein 2; NIT2_HUMAN; Nitrilase family member 2; Nitrilase homolog 2; Omega amidase NIT2; Omega-amidase NIT2; anti-NIT2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Clonality
Polyclonal
Isotype
IgG
Specificity
NIT2 Antibody detects endogenous levels of NIT2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
276
Applicable Applications for anti-NIT2 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human NIT2.
Tissue Specificity
Detected in fetal brain (at protein level). Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, prostate, spleen, thymus, prostate, testis, ovary, small intestine and colon.
Fragment
Fab Fragment
Conjugate
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Mouse kidney tissue, using NIT2 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Mouse kidney tissue, using NIT2 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-NIT2 antibody
Description: Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.
Subunit Structure: Homodimer.
Similarity: Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.
Subunit Structure: Homodimer.
Similarity: Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 30 kDa
Predicted: 31 kDa
Predicted: 31 kDa
NCBI Official Full Name
Omega-amidase NIT2
NCBI Official Synonym Full Names
nitrilase family member 2
NCBI Official Symbol
NIT2
NCBI Official Synonym Symbols
HEL-S-8a
NCBI Protein Information
omega-amidase NIT2
UniProt Protein Name
Omega-amidase NIT2
Protein Family
UniProt Gene Name
NIT2
UniProt Entry Name
NIT2_HUMAN
Uniprot Description
NIT2: a protein of unknown function. A CN hydrolase domain suggests that this protein may be involved in nitrilase-related metabolism and handling.
Protein type: EC 3.5.1.3; Hydrolase; Mitochondrial
Chromosomal Location of Human Ortholog: 3q12.2
Cellular Component: centrosome; mitochondrion; cytoplasm
Molecular Function: omega-amidase activity
Biological Process: oxaloacetate metabolic process; glutamine metabolic process; asparagine metabolic process
Research Articles on NIT2
Similar Products
Product Notes
The NIT2 nit2 (Catalog #AAA9611936) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NIT2 Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's NIT2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the NIT2 nit2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NIT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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