Western Blot (WB)
((1ug/ml) staining of Hela (A), HepG2 (B), Jurkat (C) and NIH3T3 (D) nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat NIPP1/PPP1R8 Polyclonal Antibody | anti-PPP1R8 antibody
Goat anti-NIPP1/PPP1R8 Antibody
Immunohistochemistry: In paraffin embedded Human Thymus shows speckled staining of nuclei. Recommended concentration, 2.5-3.8ug/ml.
Western Blot: Approx 40kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat and NIH3T3 (calculated MW of 38.5kDa according to Human NP_054829.2 and to Mouse NP_666266.1). Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((1ug/ml) staining of Hela (A), HepG2 (B), Jurkat (C) and NIH3T3 (D) nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Hela (A), HepG2 (B), Jurkat (C) and NIH3T3 (D) nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008]
Uniprot Description
NIPP-1: Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.4.-; Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis; Ribonuclease; Inhibitor
Chromosomal Location of Human Ortholog: 1p35.3
Cellular Component: cytoplasm; nuclear speck; nucleoplasm; nucleus; spliceosome
Molecular Function: DNA binding; endonuclease activity; protein binding; protein phosphatase type 1 regulator activity; ribonuclease E activity; RNA binding; type 1 serine/threonine specific protein phosphatase inhibitor activity
Biological Process: cell proliferation; mRNA processing; negative regulation of catalytic activity; regulation of transcription, DNA-dependent; RNA catabolic process; RNA splicing; transcription, DNA-dependent
Research Articles on PPP1R8
Similar Products
Product Notes
The PPP1R8 ppp1r8 (Catalog #AAA420033) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-NIPP1/PPP1R8 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's NIPP1/PPP1R8 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Thymus shows speckled staining of nuclei. Recommended concentration, 2.5-3.8ug/ml. Western Blot: Approx 40kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat and NIH3T3 (calculated MW of 38.5kDa according to Human NP_054829.2 and to Mouse NP_666266.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the PPP1R8 ppp1r8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EAWPGKKPTP SLLI. It is sometimes possible for the material contained within the vial of "NIPP1/PPP1R8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
