Western Blot (WB)
(Western blot analysis of NINJ1 in human brain tissue lysate with NINJ1 antibody at 1 μg/ml.)
Rabbit NINJ1 Polyclonal Antibody | anti-NINJ1 antibody
NINJ1 Antibody
Gene Names
NINJ1; NIN1; NINJURIN
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
NINJ1 antibody is affinity chromatography purified via peptide column.
Synonyms
NINJ1; Polyclonal Antibody; NINJ1 Antibody; NIN1; NINJURIN; Ninjurin-1; Nerve injury-induced protein 1; ninjurin 1; anti-NINJ1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NINJ1 antibody is human, mouse and rat reactive.
Purity/Purification
NINJ1 antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL (varies by lot)
Applicable Applications for anti-NINJ1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Application Notes
NINJ1 antibody can be used for detection of NINJ1 by Western blot at 1-2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Immunogen
NINJ1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human NINJ1.
The immunogen is located within the last 50 amino acids of NINJ1.
The immunogen is located within the last 50 amino acids of NINJ1.
Positive Control
MBS151732 (Human Brain Tissue Lysate)
Preparation and Storage
NINJ1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of NINJ1 in human brain tissue lysate with NINJ1 antibody at 1 μg/ml.)
Western Blot (WB)
(Western blot analysis of NINJ1 in human brain tissue lysate with NINJ1 antibody at 1 μg/ml.)
Immunohistochemistry (IHC)
(Immunohistochemistry of NINJ1 in mouse brain tissue with NINJ1 antibody at 5 μg/ml.)
Immunohistochemistry (IHC)
(Immunohistochemistry of NINJ1 in mouse brain tissue with NINJ1 antibody at 5 μg/ml.)
Immunofluorescence (IF)
(Immunofluorescence of NINJ1 in mouse brain tissue with NINJ1 antibody at 20 μg/ml.)
Immunofluorescence (IF)
(Immunofluorescence of NINJ1 in mouse brain tissue with NINJ1 antibody at 20 μg/ml.)
Related Product Information for anti-NINJ1 antibody
Ninjurin-1 (NINJ1) is a homophilic adhesion molecule that is strongly induced in dorsal root ganglion (DRG) and Schwann cells following nerve injury and promotes neurite outgrowth of DRG neurons (1,2), suggesting that NINJ1 may play a role in nerve regeneration. NINJ1 is transcriptionally regulated by p53 and is induced by DNA damage in a p53-dependent manner. A deficiency of NINJ1 increases p53 expression, suppresses cell proliferation, but enhances apoptosis and premature senescence in a p53-dependent manner as well as radiation-induced cell mortality (3).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 17 kDa
Observed: 16 kDa
Observed: 16 kDa
NCBI Official Full Name
ninjurin-1
NCBI Official Synonym Full Names
ninjurin 1
NCBI Official Symbol
NINJ1
NCBI Official Synonym Symbols
NIN1; NINJURIN
NCBI Protein Information
ninjurin-1; nerve injury-induced protein 1; nerve injury-induced protein-1
UniProt Protein Name
Ninjurin-1
Protein Family
UniProt Gene Name
NINJ1
UniProt Entry Name
NINJ1_HUMAN
NCBI Description
The ninjurin protein is upregulated after nerve injury both in dorsal root ganglion neurons and in Schwann cells (Araki and Milbrandt, 1996 [PubMed 8780658]). It demonstrates properties of a homophilic adhesion molecule and promotes neurite outgrowth from primary cultured dorsal root ganglion neurons.[supplied by OMIM, Aug 2009]
Uniprot Description
NINJ1: Homophilic cell adhesion molecule that promotes axonal growth. May play a role in nerve regeneration and in the formation and function of other tissues. Cell adhesion requires divalent cations. Belongs to the ninjurin family.
Protein type: Membrane protein, integral; Cell development/differentiation; Cell adhesion; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 9q22
Cellular Component: integral to membrane
Biological Process: nervous system development; tissue regeneration; cell adhesion; positive regulation of cell-matrix adhesion
Research Articles on NINJ1
Similar Products
Product Notes
The NINJ1 ninj1 (Catalog #AAA153659) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NINJ1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NINJ1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF). NINJ1 antibody can be used for detection of NINJ1 by Western blot at 1-2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Researchers should empirically determine the suitability of the NINJ1 ninj1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NINJ1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
