Rabbit anti-Human NIK Polyclonal Antibody | anti-NIK antibody

NIK, CT (MAP4K4, HGK, KIAA0687, NIK, Mitogen-activated protein kinase kinase kinase kinase 4, HPK/GCK-like kinase HGK, MAPK/ERK kinase kinase kinase 4, Nck-interacting kinase) (PE)

Cat. Num. AAA6325322

• Gene Names: MAP4K4; HGK; NIK; MEKKK4; FLH21957
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: NIK; Polyclonal Antibody; CT (MAP4K4; HGK; KIAA0687; Mitogen-activated protein kinase kinase kinase kinase 4; HPK/GCK-like kinase HGK; MAPK/ERK kinase kinase kinase 4; Nck-interacting kinase) (PE); MAP4K4; Nck-interacting kinase; anti-NIK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-NIK antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NIK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 541~571 amino acids from the C-terminal region of human HGK.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-HGK Antibody (C-term) in jurkat cell line lysates (35ug/lane). HGK(arrow) was detected using the purified Pab.)

Related Product Information for anti-NIK antibody

HGK, a member of the STE20 subfamily of Ser/Thr protein kinases, may play a role in the response to environmental stress and cytokines such as TNF-alpha. It appears to act upstream of the JUN N-terminal pathway. This protein is thought to interact with the SH3 domain of the adapter proteins Nck. HGK binds, via its CNH regulatory domain, to the N-terminal region of SPG3A. Expression appears to be ubiquitous, expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain, and isoform 4 is predominant in the liver, skeletal muscle and placenta.

Product Categories/Family for anti-NIK antibody

Antibodies; Protein Kinases

References

Wright, J.H., et al., Mol. Cell. Biol. 23(6):2068-2082 (2003). Yao, Z., et al., J. Biol. Chem. 274(4):2118-2125 (1999). Ishikawa, K., et al., DNA Res. 5(3):169-176 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 336020358
• NCBI GeneID: 9448
• NCBI Accession #: NP_001229488.1
• NCBI GenBank Nucleotide #: NM_001242559.1
• UniProt Accession #: O95819; O75172; Q9NST7
• Molecular Weight: 142,101 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 4 isoform 4
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 4
• NCBI Official Symbol: MAP4K4
• NCBI Official Synonym Symbols: HGK; NIK; MEKKK4; FLH21957
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 4; MEK kinase kinase 4; nck-interacting kinase; HPK/GCK-like kinase HGK; Ste20 group protein kinase HGK; MAPK/ERK kinase kinase kinase 4; hepatocyte progenitor kinase-like/germinal center kinase-like k
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 4
• Protein Family: Nik-related protein kinase
• UniProt Gene Name: MAP4K4
• UniProt Synonym Gene Names: HGK; KIAA0687; NIK; MEK kinase kinase 4; MEKKK 4
• UniProt Entry Name: M4K4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

HGK: a Ser/Thr kinase of the STE20 subfamily. May play a role in the response to environmental stress and cytokines such as TNF-alpha. May interact with the SH3 domain of the adapter proteins Nck. Binds, via its CNH regulatory domain, to the N- terminal region of SPG3A. Upregulated in primary tumors and tumor cell lines. Required for anchorage-independent growth and ras-dependent focus formation in multiple cell lines. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; MSN subfamily

Chromosomal Location of Human Ortholog: 2q11.2-q12

Cellular Component: cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: microvillus biogenesis; regulation of catalytic activity; regulation of JNK cascade; protein amino acid phosphorylation