Rabbit anti-Human, Rat Niemann-Pick Disease, Type C1 Gene-Like 1 Polyclonal Antibody | anti-NPC1L1 antibody

Niemann-Pick Disease, Type C1 Gene-Like 1 (NPC1L1, Niemann-Pick C1-like Protein 1, NPC1 like 1, NPC1 Niemann-Pick Disease Type C1 Gene-like 1, NPC1L1 Splice Variant, Truncated Niemann-Pick C1-like Protein 1)

Cat. Num. AAA629629

• Gene Names: NPC1L1; NPC11L1
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Niemann-Pick Disease; Type C1 Gene-Like 1; Polyclonal Antibody; Type C1 Gene-Like 1 (NPC1L1; Niemann-Pick C1-like Protein 1; NPC1 like 1; NPC1 Niemann-Pick Disease Type C1 Gene-like 1; NPC1L1 Splice Variant; Truncated Niemann-Pick C1-like Protein 1); Anti -Niemann-Pick Disease; anti-NPC1L1 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Specificity: This antibody is specific for NPC1L1 protein. Species Crossreactivity: This antibody reacts with rat and human NPC1L1 protein. There is 94% homology for mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.

• Applicable Applications for anti-NPC1L1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.
• Immunogen: A synthetic peptide made to an internal region of rat NPC1L1 (between residues 1000-1100).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-NPC1L1 antibody

Dietary consumption and intestinal cholesterol absorption contribute to plasma cholesterol levels, which factor into the risk of coronary heart disease. Niemann-Pick type C1 Like 1 protein (NPC1L1 or NPC3) is enriched in the small intestine and is found in the brush border membrane of enterocytes. It plays a critical role in absorption of intestinal cholesterol.

Product Categories/Family for anti-NPC1L1 antibody

Antibodies; Abs to Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 156231351
• NCBI GeneID: 29881
• NCBI Accession #: NP_037521.2
• NCBI GenBank Nucleotide #: NM_013389.2
• UniProt Accession #: Q9UHC9; Q17RV5; Q6R3Q4; Q9UHC8; A4D2J7; D3DVK9
• Molecular Weight: 148,728 Da
• NCBI Official Full Name: Niemann-Pick C1-like protein 1 isoform 1
• NCBI Official Synonym Full Names: NPC1-like 1
• NCBI Official Symbol: NPC1L1
• NCBI Official Synonym Symbols: NPC11L1
• NCBI Protein Information: Niemann-Pick C1-like protein 1; NPC1 (Niemann-Pick disease, type C1, gene)-like 1
• UniProt Protein Name: Niemann-Pick C1-like protein 1
• Protein Family: Niemann-Pick C1-like protein
• UniProt Gene Name: NPC1L1
• UniProt Entry Name: NPCL1_HUMAN

NCBI Description

The protein encoded by this gene is a multi-pass membrane protein. It contains a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD) which includes a YQRL motif functioning as a plasma membrane to trans-Golgi network transport signal in other proteins. This protein takes up free cholesterol into cells through vesicular endocytosis and plays a critical role in the absorption of intestinal cholesterol. It also has the ability to transport alpha-tocopherol (vitamin E). The drug ezetimibe targets this protein and inhibits the absorption of intestinal cholesterol and alpha-tocopherol. In addition, this protein may play a critical role in regulating lipid metabolism. Polymorphic variations in this gene are associated with plasma total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and coronary heart disease (CHD) risk. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

NPC1L1: Play a major role in cholesterol homeostasis. Is critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte. Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorbtion. Lack of activity leads to multiple lipid transport defects. The protein may have a function in the transport of multiple lipids and their homeostasis, and may play a critical role in regulating lipid metabolism. Interacts with RAB11A, MYO5B and RAB11FIP2. Interaction with RAB11A, MYO5B and RAB11FIP2 is required for proper transport to the plasma membrane upon cholesterol depletion. Expression is decreased in Caco-2 cells upon PPARD activation. Widely expressed. Expressed in liver. Also expressed in small intestine, pancreas, kidney, lung, pancreas, spleen, heart, gall bladder, brain, testis, stomach and muscle. Belongs to the patched family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: cytoplasmic vesicle membrane; brush border membrane; apical plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; hedgehog receptor activity; myosin V binding; drug binding; Rab GTPase binding

Biological Process: response to drug; cholesterol homeostasis; cholesterol transport; cholesterol absorption; lipoprotein metabolic process; signal transduction; cholesterol biosynthetic process; sterol transport