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Western Blot (WB) (NID1 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NID1 antibody detected the NID1 protein (arrow).)

Rabbit anti-Human NID1 Polyclonal Antibody | anti-NID1 antibody

NID1, NT (NID1, NID, Nidogen-1, Entactin) (FITC)

Gene Names
NID1; NID
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
NID1; Polyclonal Antibody; NT (NID1; NID; Nidogen-1; Entactin) (FITC); Entactin; anti-NID1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-NID1 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
NID1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 149-178 amino acids from the N-terminal region of human NID1.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NID1 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NID1 antibody detected the NID1 protein (arrow).)

Western Blot (WB) (NID1 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NID1 antibody detected the NID1 protein (arrow).)
Related Product Information for anti-NID1 antibody
This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix.
Product Categories/Family for anti-NID1 antibody
References
Nischt, R., et al. J. Invest. Dermatol. 127(3):545-554(2007). Ulazzi, L., et al. Mol. Cancer 6, 17 (2007): Colland, F., et al. Genome Res. 14(7):1324-1332(2004). Takagi, J., et al. Nature 424(6951):969-974(2003). Piecha, D., et al. Biochem. J. 367 (PT 3), 715-721 (2002).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
122,017 Da
NCBI Official Full Name
nidogen-1
NCBI Official Synonym Full Names
nidogen 1
NCBI Official Symbol
NID1
NCBI Official Synonym Symbols
NID
NCBI Protein Information
nidogen-1
UniProt Protein Name
Nidogen-1
Protein Family
UniProt Gene Name
NID1
UniProt Synonym Gene Names
NID; NID-1
UniProt Entry Name
NID1_HUMAN

NCBI Description

This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq, Jul 2008]

Uniprot Description

Nidogen-1: Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell- extracellular matrix interactions. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: extracellular matrix; extracellular region; basement membrane; basal lamina

Molecular Function: laminin-1 binding; collagen binding; proteoglycan binding; laminin binding; calcium ion binding

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; cell-matrix adhesion; glomerular basement membrane development

Research Articles on NID1

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Product Notes

The NID1 nid1 (Catalog #AAA6325304) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NID1, NT (NID1, NID, Nidogen-1, Entactin) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NID1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NID1 nid1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NID1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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