Rabbit Nicastrin Polyclonal Antibody | anti-NCSTN antibody

Nicastrin Antibody

Cat. Num. AAA9413352

• Gene Names: NCSTN; ATAG1874
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: Nicastrin; Polyclonal Antibody; Nicastrin Antibody; Anterior pharynx defective 2; APH-2; anti-NCSTN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 709

• Applicable Applications for anti-NCSTN antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 18 amino acid peptide from near the center of human Nicastrin.
• Target Name: Nicastrin
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Nicastrin in human brain tissue lysate with Nicastrin antibody at (A) 0.5 and (B) 1ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of Nicastrin in human brain tissue with Nicastrin antibody at 2.5 ug/mL.)

Related Product Information for anti-NCSTN antibody

Nicastrin, in addition to presenilin, PEN2, and APH-1 forms the gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease. Nicastrin is also thought to be involved in cell proliferation and signaling, especially in regards to activation of Notch receptors as loss of Nicastrin expression results in mouse embryonic lethality.

Product Categories/Family for anti-NCSTN antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 24638433
• NCBI GeneID: 23385
• NCBI Accession #: NP_056146
• NCBI GenBank Nucleotide #: NM_015331.2
• Molecular Weight: 76,744 Da
• NCBI Official Full Name: nicastrin isoform 1
• NCBI Official Synonym Full Names: nicastrin
• NCBI Official Symbol: NCSTN
• NCBI Official Synonym Symbols: ATAG1874
• NCBI Protein Information: nicastrin
• UniProt Protein Name: Nicastrin
• Protein Family: Nicastrin
• UniProt Gene Name: NCSTN
• UniProt Synonym Gene Names: KIAA0253
• UniProt Entry Name: NICA_HUMAN

NCBI Description

This gene encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21. Alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Feb 2014]

Uniprot Description

nicastrin: Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta- amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex. Component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. Such minimal complex is sufficient for secretase activity, although other components may exist. Binds to proteolytic processed C-terminal fragments C83 and C99 of the amyloid precursor protein (APP). Constitutively expressed in neural cells. Widely expressed. Belongs to the nicastrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q22-q23

Cellular Component: Golgi apparatus; focal adhesion; membrane; integral to plasma membrane; lysosomal membrane; endoplasmic reticulum; plasma membrane; integral to membrane; melanosome

Molecular Function: protein binding; endopeptidase activity

Biological Process: positive regulation of catalytic activity; axon guidance; extracellular matrix organization and biogenesis; Notch signaling pathway; membrane protein intracellular domain proteolysis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; myeloid cell homeostasis; membrane protein ectodomain proteolysis; Notch receptor processing; proteolysis; T cell proliferation; extracellular matrix disassembly; amyloid precursor protein catabolic process; epithelial cell proliferation; beta-amyloid metabolic process; ephrin receptor signaling pathway; protein processing

Disease: Acne Inversa, Familial, 1