Rabbit Nibrin, p95 Polyclonal Antibody | anti-NBN antibody

Nibrin, p95 (Nijmegen Breakage Syndrome, NBS1)

Cat. Num. AAA622025

• Gene Names: NBN; ATV; NBS; P95; NBS1; AT-V1; AT-V2
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Protein A and peptide affinity chromatography.
• Synonyms: Nibrin; p95; Polyclonal Antibody; p95 (Nijmegen Breakage Syndrome; NBS1); Anti -Nibrin; anti-NBN antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of total human p95/NBS1 protein. Species crossreactivity: mouse, rat and monkey.
• Purity/Purification: Affinity Purified; Protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-NBN antibody: Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic peptide (KLH coupled) corresponding to C-terminal residues of human p95/NBS1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunocytochemistry (ICC)

(Immunocytochemical staining of Hela cells, using p95/NBS1 Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of p95/NBS1 in paraffin-embedded human breast carcinoma showing nuclear localization, using p95/NBS1 Antibody.)

Western Blot (WB)

(Western blot analysis of extracts from COS, PC12, NIH3T3, Hela and MvILu cells, using p95/NBS1 Antibody.)

Related Product Information for anti-NBN antibody

The Nijmegen breakage syndrome (NBS) is characterized by defects in cell cycle checkpoints, growth retardation, an increased propensity for cancer and sensitivity to ionizing radiation (1). Repair of DNA double-strand breaks by radiation is dependent on a multifunctional complex containing Rad50, Mre11 and the NBS1 gene product p95/NBS1 (also called p95 or nibrin) (2). p95/NBS1 is a protein with a Forkheadassociated domain and a carboxy-terminal repeat frequently found in cell cycle regulatory and DNA repair proteins (1,3). The overlap between clinical and cellular phenotypes in ataxia telangiectasia (AT) and NBS suggests that AT-mutated (ATM) and p95/NBS1 may function in the same biochemical pathway. Recent research has shown that ATM interacts with and phosphorylates p95/NBS1 on Ser343 after IR (4-7).

Product Categories/Family for anti-NBN antibody

Antibodies; Abs to Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 33356172
• NCBI GeneID: 4683
• NCBI Accession #: NP_002476.2
• NCBI GenBank Nucleotide #: NM_002485.4
• UniProt Accession #: O60934; O60672; Q32NF7; Q53FM6; Q63HR6; Q7LDM2; B2R626; B2RNC5
• Molecular Weight: 84,959 Da
• NCBI Official Full Name: nibrin
• NCBI Official Synonym Full Names: nibrin
• NCBI Official Symbol: NBN
• NCBI Official Synonym Symbols: ATV; NBS; P95; NBS1; AT-V1; AT-V2
• NCBI Protein Information: nibrin; cell cycle regulatory protein p95; Nijmegen breakage syndrome 1 (nibrin); p95 protein of the MRE11/RAD50 complex
• UniProt Protein Name: Nibrin
• Protein Family: Nibrin
• UniProt Gene Name: NBN
• UniProt Synonym Gene Names: NBS; NBS1; P95
• UniProt Entry Name: NBN_HUMAN

NCBI Description

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008]

Uniprot Description

NBS1: a member of the MRE11/RAD50 double-strand break repair complex. Involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Mutation results in the Nijmegen breakage syndrome (NBS), an autosomal recessive chromosomal instability syndrome.

Protein type: DNA repair, damage; Cell cycle regulation

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: nucleoplasm; PML body; Mre11 complex; nuclear chromosome, telomeric region; nucleolus; replication fork; nucleus; nuclear inclusion body

Molecular Function: ATP-dependent DNA helicase activity; protein binding; damaged DNA binding; protein N-terminus binding; transcription factor binding

Biological Process: DNA damage response, signal transduction by p53 class mediator; mitotic cell cycle checkpoint; isotype switching; positive regulation of kinase activity; DNA repair; positive regulation of protein amino acid autophosphorylation; double-strand break repair via homologous recombination; DNA duplex unwinding; regulation of DNA replication initiation; cell proliferation; meiotic cell cycle; mitotic cell cycle G2/M transition DNA damage checkpoint; double-strand break repair; DNA damage checkpoint; blastocyst growth; cell cycle arrest; neuromuscular process controlling balance; telomere maintenance

Disease: Leukemia, Acute Lymphoblastic; Nijmegen Breakage Syndrome; Aplastic Anemia