Western Blot (WB)
(Western Blot analysis in NCI-H292 cell line lysates (35ug/lane) usingMBS6004194. This demonstrates that MBS6004194 detected the NIACR1 protein (arrow).)
Rabbit anti-Human NIACR1 Polyclonal Antibody | anti-NIACR1 antibody
NIACR1, ID (HCAR2, GPR109A, HCA2, HM74A, NIACR1, Hydroxycarboxylic acid receptor 2, G-protein coupled receptor 109A, G-protein coupled receptor HM74A, Niacin receptor 1, Nicotinic acid receptor) (APC)
Gene Names
HCAR2; HCA2; HM74a; HM74b; PUMAG; NIACR1; Puma-g; GPR109A
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
NIACR1; Polyclonal Antibody; ID (HCAR2; GPR109A; HCA2; HM74A; Hydroxycarboxylic acid receptor 2; G-protein coupled receptor 109A; G-protein coupled receptor HM74A; Niacin receptor 1; Nicotinic acid receptor) (APC); HCAR2; Nicotinic acid receptor; anti-NIACR1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human NIACR1.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-NIACR1 antibody
Western Blot (WB), FLISA
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 200-228 of human NIACR1. Species sequence homology: mouse
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in NCI-H292 cell line lysates (35ug/lane) usingMBS6004194. This demonstrates that MBS6004194 detected the NIACR1 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in NCI-H292 cell line lysates (35ug/lane) usingMBS6004194. This demonstrates that MBS6004194 detected the NIACR1 protein (arrow).)
Related Product Information for anti-NIACR1 antibody
NIACR1 acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.
Product Categories/Family for anti-NIACR1 antibody
References
1. Li, X., et al. Biochem. Pharmacol. 80(9):1450-1457(2010). 2. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 3. Li, G., et al. J. Biol. Chem. 285(29):22605-22618(2010). 4. Mandrika, I., et al. Biochem. Biophys. Res. Commun. 395(2):281-287(2010). 5. Shen, H.C., et al. J. Med. Chem. 53(6):2666-2670(2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,850 Da
NCBI Official Full Name
hydroxycarboxylic acid receptor 2
NCBI Official Synonym Full Names
hydroxycarboxylic acid receptor 2
NCBI Official Symbol
HCAR2
NCBI Official Synonym Symbols
HCA2; HM74a; HM74b; PUMAG; NIACR1; Puma-g; GPR109A
NCBI Protein Information
hydroxycarboxylic acid receptor 2; niacin receptor 1; nicotinic acid receptor; G protein-coupled receptor 109A; G-protein coupled receptor 109A; G protein-coupled receptor HM74a; G-protein coupled receptor HM74A; hydroxy-carboxylic acid receptor 2
UniProt Protein Name
Hydroxycarboxylic acid receptor 2
UniProt Gene Name
HCAR2
UniProt Synonym Gene Names
GPR109A; HCA2; HM74A; NIACR1
Uniprot Description
Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.
Research Articles on NIACR1
Similar Products
Product Notes
The NIACR1 hcar2 (Catalog #AAA6325291) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NIACR1, ID (HCAR2, GPR109A, HCA2, HM74A, NIACR1, Hydroxycarboxylic acid receptor 2, G-protein coupled receptor 109A, G-protein coupled receptor HM74A, Niacin receptor 1, Nicotinic acid receptor) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NIACR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NIACR1 hcar2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NIACR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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