Rabbit anti-Human NHLH1 Polyclonal Antibody | anti-NHLH1 antibody

NHLH1, ID (NHLH1, BHLHA35, HEN1, Helix-loop-helix protein 1, Class A basic helix-loop-helix protein 35, Nescient helix loop helix 1) (APC)

Cat. Num. AAA6325247

• Gene Names: NHLH1; HEN1; NSCL; NSCL1; bHLHa35
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NHLH1; Polyclonal Antibody; ID (NHLH1; BHLHA35; HEN1; Helix-loop-helix protein 1; Class A basic helix-loop-helix protein 35; Nescient helix loop helix 1) (APC); Nescient helix loop helix 1; anti-NHLH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-NHLH1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NHLH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-67 amino acids from the Central region of human NHLH1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NHLH1 Antibody (Center) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the NHLH1 antibody detected the NHLH1 protein (arrow).)

Product Categories/Family for anti-NHLH1 antibody

Antibodies; Transcription Factors

References

Manetopoulos, C., et al. Biochem. Biophys. Res. Commun. 307(4):891-899(2003). Bao, J., et al. Development 127(2):425-435(2000). Lipkowitz, S., et al. J. Biol. Chem. 267(29):21065-21071(1992) Brown, L., et al. Proc. Natl. Acad. Sci. U.S.A. 89(18):8492-8496(1992) Begley, C.G., et al. Proc. Natl. Acad. Sci. U.S.A. 89(1):38-42(1992).

NCBI and Uniprot Product Information

• NCBI GI #: 5031943
• NCBI GeneID: 4807
• NCBI Accession #: NP_005589.1
• NCBI GenBank Nucleotide #: NM_005598.3
• UniProt Accession #: Q02575
• Molecular Weight: 14,618 Da
• NCBI Official Full Name: helix-loop-helix protein 1
• NCBI Official Synonym Full Names: nescient helix loop helix 1
• NCBI Official Symbol: NHLH1
• NCBI Official Synonym Symbols: HEN1; NSCL; NSCL1; bHLHa35
• NCBI Protein Information: helix-loop-helix protein 1; HEN-1; NSCL-1; class A basic helix-loop-helix protein 35
• UniProt Protein Name: Helix-loop-helix protein 1
• Protein Family: Helix-loop-helix protein
• UniProt Gene Name: NHLH1
• UniProt Synonym Gene Names: BHLHA35; HEN1; HEN-1; bHLHa35; NSCL-1
• UniProt Entry Name: HEN1_HUMAN

NCBI Description

The helix-loop-helix (HLH) proteins are a family of putative transcription factors, some of which have been shown to play an important role in growth and development of a wide variety of tissues and species. Four members of this family have been clearly implicated in tumorigenesis via their involvement in chromosomal translocations in lymphoid tumors: MYC (MIM 190080), LYL1 (MIM 151440), E2A (MIM 147141), and SCL (MIM 187040).[supplied by OMIM, Nov 2002]

Uniprot Description

NHLH1: May serve as DNA-binding protein and may be involved in the control of cell-type determination, possibly within the developing nervous system.

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: nucleus

Molecular Function: protein dimerization activity

Biological Process: central nervous system development; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; cell differentiation