anti-Rat NHE2 Polyclonal Antibody | anti-NHE2 antibody
Anti - NHE2 (Na, H Exchanger 2)
Gene Names
Nhe2; CG9255; CG9256; DmelCG9256; DmNHE2; nhe2; NHE2
Reactivity
Rat
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
NHE2; Polyclonal Antibody; Anti - NHE2 (Na; H Exchanger 2); NHE2 (Na; anti-NHE2 antibody
Host
Host: Chicken
Source: Rat
Source: Rat
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgY
Specificity
Recognizes rat NHE2. No significant homology exists with other NHE isoforms (NHE1, 3-5). Species sequence homology
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, 0.05% sodium azide.
Supplied as a liquid, 0.05% sodium azide.
Sequence Length
1322
Applicable Applications for anti-NHE2 antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA and Western Blot. Other applications not tested.
Dilution: ELISA: 1:100,000 using 5-10ng control peptide/well. Control peptide is N2016-07.
Western Blot: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Dilution: ELISA: 1:100,000 using 5-10ng control peptide/well. Control peptide is N2016-07.
Western Blot: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Immunogen
Synthetic peptide corresponding to 20aa sequence near C-terminus of rat NHE2.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-NHE2 antibody
Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. Five NHE isoforms (NHE1-5) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The COOH-terminals of NHE1, NHE2 and NHE3 are intracellular.
Product Categories/Family for anti-NHE2 antibody
References
• 1. Wang Z et al (1993) J Biol Chem. 268, 11925; Collins JF et al (1990) PNAS 90, 9398; Ghishan FK et al (1995) Genomics 30, 25; Chris Yun CH et al (1995) Am J Physiol. 269, G1-G11 (Review); Josette N and Pouyssegur J (1995) Am J Physiol. 268, C283-C296 (review); Brookstein C et al (1994) J Biol. Chem. 269, 29704.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
142,332 Da
NCBI Official Full Name
Na[+]/H[+] hydrogen exchanger 2, isoform G
NCBI Official Synonym Full Names
Na[+]/H[+] hydrogen exchanger 2
NCBI Official Symbol
Nhe2
NCBI Official Synonym Symbols
CG9255; CG9256; DmelCG9256; DmNHE2; nhe2; NHE2
NCBI Protein Information
CG9256 gene product from transcript CG9256-RE; Na[+]/H[+] hydrogen exchanger 2, isoform E; CG9256-PE; Nhe2-PE; Na[+]/H[+] hydrogen exchanger 2, isoform A; CG9256-PA; Nhe2-PA; Na[+]/H[+] hydrogen exchanger 2, isoform G; CG9256-PG; Nhe2-PG; Na[+]/H[+] hydrogen exchanger 2, isoform B; CG9256-PB; Nhe2-PB; Na[+]/H[+] hydrogen exchanger 2, isoform C; CG9256-PC; Nhe2-PC; Na[+]/H[+] hydrogen exchanger 2, isoform D; CG9256-PD; Nhe2-PD; Na[+]/H[+] hydrogen exchanger 2, isoform F; CG9256-PF; Nhe2-PF
UniProt Protein Name
Sodium/hydrogen exchanger
UniProt Gene Name
Nhe2
UniProt Entry Name
Q9VIF9_DROME
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Product Notes
The NHE2 nhe2 (Catalog #AAA618569) is an Antibody produced from Host: Chicken Source: Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti - NHE2 (Na, H Exchanger 2) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NHE2 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA and Western Blot. Other applications not tested. Dilution: ELISA: 1:100,000 using 5-10ng control peptide/well. Control peptide is N2016-07. Western Blot: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the NHE2 nhe2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NHE2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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