Rabbit anti-Human NGFR Polyclonal Antibody | anti-NGFR antibody

NGFR Antibody (C-term)

Cat. Num. AAA9215684

• Gene Names: NGFR; CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
• Reactivity: Human
• Applications: Western Blot
• Synonyms: NGFR; Polyclonal Antibody; NGFR Antibody (C-term); Purified Rabbit Polyclonal Antibody (Pab); Tumor necrosis factor receptor superfamily member 16; Gp80-LNGFR; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; NGF receptor; p75 ICD; CD271; TNFRSF16; anti-NGFR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This NGFR antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 276-310 amino acids from the C-terminal region of human NGFR.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 427

• Applicable Applications for anti-NGFR antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-NGFR Antibody (C-term) at 1:2000 dilution + SW480 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 45 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-NGFR antibody

Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver.

Product Categories/Family for anti-NGFR antibody

Crown Antibodies; Neuroscience; Stem Cells

References

Johnson D.,et al.Cell 47:545-554(1986). Ota T.,et al.Nat. Genet. 36:40-45(2004). Zody M.C.,et al.Nature 440:1045-1049(2006). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Sehgal A.,et al.Mol. Cell. Biol. 8:3160-3167(1988).

NCBI and Uniprot Product Information

• NCBI GI #: 4505393
• NCBI GeneID: 4804
• NCBI Accession #: NP_002498.1
• NCBI GenBank Nucleotide #: NM_002507.3
• UniProt Accession #: P08138; B2R961; B4E096
• Molecular Weight: 45183
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 16
• NCBI Official Synonym Full Names: nerve growth factor receptor
• NCBI Official Symbol: NGFR
• NCBI Official Synonym Symbols: CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 16
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 16
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: NGFR
• UniProt Synonym Gene Names: TNFRSF16; NGF receptor
• UniProt Entry Name: TNR16_HUMAN

NCBI Description

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq, Jul 2008]

Uniprot Description

NGFR: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Homodimer; disulfide-linked. Interacts with p75NTR- associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3. Interacts with KIDINS220 and NTRK1. Can form a ternary complex with NTRK1 and KIDINS220 and this complex is affected by the expression levels of KIDINS220. An increase in KIDINS220 expression leads to a decreased association of NGFR and NTRK1. Interacts with NTRK2; may regulate the ligand specificity of the NTRK2 receptor. Interacts with LINGO1.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 17q21-q22

Cellular Component: nucleoplasm; Golgi apparatus; cell surface; rough endoplasmic reticulum; integral to plasma membrane; plasma membrane; extracellular region; nuclear envelope; perikaryon; clathrin-coated endocytic vesicle; cytosol; endosome; external side of plasma membrane; lipid raft

Molecular Function: protein binding; signal transducer activity; transmembrane receptor activity; ubiquitin protein ligase binding; death receptor activity; beta-amyloid binding; neurotrophin TRKA receptor binding; nerve growth factor binding; receptor activity; Rab GTPase binding

Biological Process: axon guidance; detection of temperature stimulus; central nervous system development; nerve growth factor receptor signaling pathway; negative regulation of fibroblast growth factor receptor signaling pathway; positive regulation of apoptosis; response to lipopolysaccharide; regulation of caspase activity; regulation of axonogenesis; sensory perception of pain; glucose homeostasis; positive regulation of synaptic transmission, glutamatergic; negative regulation of mitochondrial depolarization; negative regulation of cell cycle; intracellular protein transport; positive regulation of fibroblast proliferation; positive regulation of MAPKKK cascade; response to wounding; negative regulation of axonogenesis; negative regulation of neuron apoptosis; circadian regulation of gene expression; positive regulation of myelination; positive regulation of synaptic transmission, cholinergic; hair follicle morphogenesis; membrane protein intracellular domain proteolysis; positive regulation of Rho protein signal transduction; positive regulation of odontogenesis of dentine-containing teeth; negative regulation of hair follicle development; positive regulation of protein kinase B signaling cascade; positive regulation of axonogenesis; nerve development; negative regulation of apoptosis