Western Blot (WB)
(NFYB Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NFYB antibody detected the NFYB protein (arrow).)
Rabbit NFYB Polyclonal Antibody | anti-NFYB antibody
NFYB Antibody (N-term)
Western Blot (WB)
(NFYB Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NFYB antibody detected the NFYB protein (arrow).)
Western Blot (WB)
(NFYB Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NFYB antibody detected the NFYB protein (arrow).)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with NFYB Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFYB primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFYB immunoreactivity is localized to nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with NFYB Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFYB primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFYB immunoreactivity is localized to nucleus significantly.)
trimeric complex, forming a highly conserved transcription factor
that binds with high specificity to CCAAT motifs in the promoter
regions in a variety of genes. This gene product, subunit B, forms
a tight dimer with the C subunit, a prerequisite for subunit A
association. The resulting trimer binds to DNA with high
specificity and affinity. Subunits B and C each contain a
histone-like motif. Observation of the histone nature of these
subunits is supported by two types of evidence; protein sequence
alignments and experiments with mutants.
Kang, J.H., et al. J. Cell. Biochem. 104(4):1192-1203(2008)
Raju, C.S., et al. Mol. Biol. Cell 19(7):3008-3019(2008)
Di Agostino, S., et al. Cancer Cell 10(3):191-202(2006)
Kahle, J., et al. Mol. Cell. Biol. 25(13):5339-5354(2005)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008]
Uniprot Description
NFYB: Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Belongs to the NFYB/HAP3 subunit family.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 12q22-q23
Cellular Component: nucleoplasm; CCAAT-binding factor complex; nucleus
Molecular Function: protein binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; protein complex binding; transcription factor activity
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; cellular lipid metabolic process
Research Articles on NFYB
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Product Notes
The NFYB nfyb (Catalog #AAA9204987) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 17-46. The NFYB Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's NFYB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF). WB~~1:1000. Researchers should empirically determine the suitability of the NFYB nfyb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFYB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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