Rabbit anti-Human NFIB Polyclonal Antibody | anti-NFIB antibody

NFIB, ID (NFIB, Nuclear factor 1 B-type, CCAAT-box-binding transcription factor, Nuclear factor I/B, TGGCA-binding protein) (FITC)

Cat. Num. AAA6325007

• Gene Names: NFIB; CTF; MACID; NF1-B; NFI-B; NFIB2; NFIB3; NF-I/B; NFI-RED; HMGIC/NFIB
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NFIB; Polyclonal Antibody; ID (NFIB; Nuclear factor 1 B-type; CCAAT-box-binding transcription factor; Nuclear factor I/B; TGGCA-binding protein) (FITC); TGGCA-binding protein; anti-NFIB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 420

• Applicable Applications for anti-NFIB antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NFIB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-283 amino acids from the Central region of human NFIB.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NFIB Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NFIB antibody detected the NFIB protein (arrow).)

Related Product Information for anti-NFIB antibody

NFIB recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Product Categories/Family for anti-NFIB antibody

Antibodies; Transcription Factors

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Persson, M., et al. Proc. Natl. Acad. Sci. U.S.A. 106(44):18740-18744(2009). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Pierron, A., et al. Cancer Genet. Cytogenet. 195(1):66-70(2009). Le Clerc, S., et al. J. Infect. Dis. 200(8):1194-1201(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 93004092
• NCBI GeneID: 4781
• NCBI Accession #: NP_005587.2
• NCBI GenBank Nucleotide #: NP_005587.2
• UniProt Accession #: O00712
• NCBI Official Full Name: nuclear factor 1 B-type isoform 3
• NCBI Official Synonym Full Names: nuclear factor I B
• NCBI Official Symbol: NFIB
• NCBI Official Synonym Symbols: CTF; MACID; NF1-B; NFI-B; NFIB2; NFIB3; NF-I/B; NFI-RED; HMGIC/NFIB
• NCBI Protein Information: nuclear factor 1 B-type
• UniProt Protein Name: Nuclear factor 1 B-type
• Protein Family: Nuclear factor
• UniProt Gene Name: NFIB
• UniProt Synonym Gene Names: NF1-B; Nuclear factor 1/B; CTF; NF-I/B; NFI-B
• UniProt Entry Name: NFIB_HUMAN

Uniprot Description

NFI-B: Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Belongs to the CTF/NF-I family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: DNA binding; double-stranded DNA binding

Biological Process: transcription from RNA polymerase II promoter; pontine nucleus development; glial cell differentiation; anterior commissure morphogenesis; chondrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; DNA replication; negative regulation of DNA binding; hindbrain development